Genetic control of levels of murine kidney glucuronidase mRNA in response to androgen.
Genetic control of levels of murine kidney glucuronidase mRNA in response to androgen.
复制标题
小鼠肾葡萄糖醛酸酶 mRNA 水平对雄激素反应的遗传控制。
DOI：
10.1073/pnas.80.24.7596
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发表时间：
1983
影响因子：
11.1
通讯作者：
Ganschow,RE
中科院分区：
文献类型：
--
作者：
Palmer,R;Gallagher,PM;Boyko,WL;Ganschow,RE
一种顺式作用遗传元件，命名为Gus-r，调节肾小管细胞中雄激素诱导的鼠葡糖醛酸糖苷酶（EC 3.2.1.31）合成速率，并与葡糖醛酸糖苷酶结构基因Gus-s紧密相连。为了研究这种调节的分子机制，我们在质粒pBR 322中克隆了葡萄糖醛酸酶特异性cDNA序列。该克隆的DNA已被用作印迹杂交分析中的探针，以确定Gus-r对肾葡萄糖醛酸苷酶合成的雄激素反应性的控制是否在葡萄糖醛酸苷酶mRNA的水平或可翻译性上发挥作用。这些研究中出现了三个重要的观察结果：（i）葡萄糖醛酸苷酶mRNA以约2，800个核苷酸的单一大小类别存在;（ii）葡萄糖醛酸苷酶合成的雄激素刺激与葡萄糖醛酸苷酶mRNA水平直接相关;（iii）响应雄激素积累的肾脏葡萄糖醛酸苷酶mRNA水平的品系差异受Gus-r等位基因控制。因此，Gus-r通过控制可用于翻译的葡萄糖醛酸苷酶mRNA的量来调节葡萄糖醛酸苷酶合成的雄激素响应性，并且是调节特定mRNA的激素响应性的顺式作用遗传元件。
A cis-acting genetic element, designated Gus-r, regulates the androgen-induced rates of murine glucuronidase (EC 3.2.1.31) synthesis in kidney tubule cells and is tightly linked to the glucuronidase structural gene, Gus-s. To investigate the molecular mechanism underlying this regulation, we have cloned a glucuronidase-specific cDNA sequence in plasmid pBR322. This cloned DNA has been utilized as a probe in blot hybridization analyses to determine whether the control of androgen responsiveness of kidney glucuronidase synthesis by Gus-r is exerted over the level or the translatability of glucuronidase mRNA. Three important observations emerged from these studies: (i) glucuronidase mRNA exists as a single size class of approximately 2,800 nucleotides; (ii) androgen stimulation of glucuronidase synthesis is directly related to the level of glucuronidase mRNA; and (iii) strain differences in levels of kidney glucuronidase mRNA accumulated in response to androgen are controlled by alleles of Gus-r. Thus, Gus-r regulates the androgen responsiveness of glucuronidase synthesis by controlling the amount of glucuronidase mRNA available for translation and is a cis-acting genetic element that regulates the hormonal responsiveness of a specific mRNA.