Reading and Writing Digital Information in TNA
Reading and Writing Digital Information in TNA
复制标题
在 TNA 中读取和写入数字信息
DOI：
10.1021/acssynbio.0c00361
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发表时间：
2020
影响因子：
4.7
通讯作者：
Chaput, John C.
中科院分区：
文献类型：
--
作者：
Yang, Kefan;McCloskey, Cailen M.;Chaput, John C.
DNA已成为一种流行的低能量、高密度信息存储的软材料，但它很容易受到环境中氧化、pH、温度和核酸酶的破坏。在这里，我们描述了一种基于 α-l-苏呋喃糖基核酸 (TNA) 非自然遗传框架的用于数据存档的新分子化学型。使用简单的遗传编码策略，23 KB 的数字信息被存储在 DNA 引发的 TNA 寡核苷酸中，并在暴露于破坏等效 DNA 信息的生物核酸酶后以完美的精度恢复。我们认为，这些结果通过提供防止核酸酶消化引起的信息丢失的保护措施，扩展了核酸作为低能量、高密度信息存储的软材料的能力。
DNA has become a popular soft material for low energy, high-density information storage, but it is susceptible to damage through oxidation, pH, temperature, and nucleases in the environment. Here, we describe a new molecular chemotype for data archiving based on the unnatural genetic framework of α-l-threofuranosyl nucleic acid (TNA). Using a simple genetic coding strategy, 23 kilobytes of digital information were stored in DNA-primed TNA oligonucleotides and recovered with perfect accuracy after exposure to biological nucleases that destroyed equivalent DNA messages. We suggest that these results extend the capacity for nucleic acids to function as a soft material for low energy, high-density information storage by providing a safeguard against information loss caused by nuclease digestion.