Evaluation of renal hypoxia in diabetic mice by BOLD MRI.
Evaluation of renal hypoxia in diabetic mice by BOLD MRI.
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DOI：
10.1097/rli.0b013e3181ec9b02
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发表时间：
2010-12
影响因子：
6.7
通讯作者：
Batlle D
中科院分区：
文献类型：
--
作者：
Prasad P;Li LP;Halter S;Cabray J;Ye M;Batlle D
肾缺氧已被认为是糖尿病肾病的病理生理学特征，但很难在体内证明，特别是在糖尿病小鼠模型中。这项工作的目的是检查血氧水平依赖性 (BOLD) 磁共振成像 (MRI) 的敏感性，以评估糖尿病肾病小鼠模型（db/db 小鼠）体内肾氧合。肾脏 BOLD MRI 研究是在 3.0 T 扫描仪上进行的，使用多重梯度回波序列和定制设计的表面线圈来获取 T2* 加权图像。研究在 10 周龄 db/db 小鼠（n = 7）和 db/m 对照小鼠（n = 6）中进行。 R2* 是组织脱氧血红蛋白浓度的量度，较高的 R2* 值与缺氧有关。 db/db 小鼠具有较高的髓质（43.1 ± 5.1 s−1 与 32.3 ± 3.7 s−1，P = 0.001）和皮质 R2*（31.7 ± 3.1 s−1 与 27.1 ± 4.1 s−1，P = 0.04）值。使用哌莫硝唑染色作为肾脏缺氧的标志物，在10周龄db/db小鼠的肾脏切片中，与10周龄db/m小鼠相比，皮质和髓质均没有显着差异（皮质：db/db 2.14±0.05 vs db/m 2.02±0.28，髓质：db/db 2.81±0.08 vs db/m）。分贝/米2.6±0.08）。 15 周龄再次扫描时，db/db 小鼠的皮质和髓质缺氧情况进一步加剧。该报告表明，肾脏 BOLD MRI 是一种在糖尿病肾病小鼠模型中体内评估肾缺氧的敏感方法，可以记录随着时间的推移进行性肾缺氧。 BOLD MRI 可能有助于监测可改善糖尿病肾脏组织缺氧的治疗干预措施。
Renal hypoxia has been proposed to be a pathophysiologic feature of diabetic kidney disease but it has been difficult to demonstrate in vivo, particularly in mouse models of diabetes. The objective of this work was to examine the sensitivity of blood oxygen level-dependent (BOLD) magnetic resonance imaging (MRI) to assess renal oxygenation in vivo in a mouse model of diabetic kidney disease, the db/db mice. Kidney BOLD MRI studies were performed on a 3.0 T scanner using multiple gradient echo sequence with a custom-designed surface coil to acquire T2*-weighted images. Studies were performed in 10-week-old db/db mice (n = 7) and db/m controls (n = 6). R2* is a measure of the tissue deoxyhemoglobin concentration and higher values of R2* are associated with hypoxia. The db/db mice had higher medullary (43.1 ± 5.1 s−1 vs. 32.3 ± 3.7 s−1, P = 0.001) and cortical R2* (31.7 ± 3.1 s−1 vs. 27.1 ± 4.1 s−1, P = 0.04) values. Using pimonidazole staining as a marker of kidney hypoxia, in kidney sections from 10-week-old db/db mice neither cortex nor medulla had significant differences as compared with 10-week-old db/m mice (cortex: db/db 2.14 ± 0.05 vs. db/m 2.02 ± 0.28, medulla: db/db 2.81 ± 0.08 vs. db/m 2.6 ± 0.08). The db/db mice demonstrated further increased cortical and medullary hypoxia when scanned again at 15 weeks of age. The report shows that renal BOLD MRI is a sensitive method for the in vivo evaluation of renal hypoxia in a mouse model of diabetic kidney disease where progressive renal hypoxia can be documented over time. BOLD MRI may be useful to monitor therapeutic interventions that may improve tissue hypoxia in the diabetic kidney.