Analysis of Pirlimycin Residues in Beef Muscle, Milk, and Honey by a Biotin-Streptavidin-Amplified Enzyme-Linked Immunosorbent Assay
Analysis of Pirlimycin Residues in Beef Muscle, Milk, and Honey by a Biotin-Streptavidin-Amplified Enzyme-Linked Immunosorbent Assay
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DOI：
10.1021/acs.jafc.5b05711
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发表时间：
2016-01-13
影响因子：
6.1
通讯作者：
Xu, Gaixia
中科院分区：
文献类型：
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作者：
Jiang, Wenxiao;Beier, Ross C.;Xu, Gaixia
兽药残留污染食品是一个全球性的公共卫生问题，需要持续监测。在这项研究中，我们开发了一种生物素-链亲和素扩增ELISA (BA-ELISA)，使用生产的单克隆抗体检测牛肉肌肉、牛奶和蜂蜜中的匹利霉素残留。BA-ELISA对缓冲液中吡霉素的IC50值为1.6 ng/mL，灵敏度比传统elisa提高3倍。优化后的BA-ELISA可用于定量测定牛肉肌肉、牛奶和蜂蜜中痕量的吡林霉素残留。该方法的检出限（lod）分别为牛肉肌4.45 μ g/kg、牛奶1.65 μ g/L、蜂蜜2.75 μ g/kg。BA-ELISA的平均回收率。变异系数为5.3% ~ 13.5%。建立的BA-ELISA方法经LC-MS/MS验证，可用于吡利霉素残留的常规筛选分析。
Food contamination by veterinary drug residues is a worldwide public health concern and requires continuous monitoring. In this study, we developed a biotin-streptavidin-amplified ELISA (BA-ELISA) using a produced monoclonal antibody for detecting pirlimycin residues in beef muscle, milk, and honey. The IC50 value of the BA-ELISA was 1.6 ng/mL for pirlimycin in buffer, and the sensitivity was improved 3 times compared to traditional ELISAs. The optimized BA-ELISA can be used to quantitate trace amounts of pirlimycin residues in beef muscle, milk, and honey. This method had limits of detection (LODs) of 4.45 mu g/kg in beef muscle, 1.65 mu g/L in milk, and 2.75 mu g/kg in honey. The average recovery of the BA-ELISA. ranged from 78 to 97%, and the coefficient of variation ranged from 5.3 to 13.5%. The developed BA-ELISA method was validated using LC-MS/MS, and the BA-ELISA can be used for routine screening analysis of pirlimycin residues.