CONJUGATION IN TETRAHYMENA-THERMOPHILA - A TEMPORAL ANALYSIS OF CYTOLOGICAL STAGES
CONJUGATION IN TETRAHYMENA-THERMOPHILA - A TEMPORAL ANALYSIS OF CYTOLOGICAL STAGES
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DOI：
10.1016/0014-4827(82)90172-0
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发表时间：
1982-01-01
影响因子：
3.7
通讯作者：
BRUNS, PJ
中科院分区：
文献类型：
--
作者：
MARTINDALE, DW;ALLIS, CD;BRUNS, PJ
通过细胞学上观察到的细胞核形态和位置的变化，确定了嗜热t细胞接合阶段的时间进程和同步性。确定了50%的配对进入或通过特定阶段所需的时间以及每个阶段的持续时间。在配对过程中，它们的相对同步性是通过将一个阶段中发现的种群的最大百分比与该阶段的持续时间相关联来实现的。在本研究条件下，对之间的同步性程度很高，在共轭的最初9小时内，同步性几乎没有下降。虽然在不同的交配中可以看到同步程度的一些变化，但在每个细胞学阶段的持续时间上几乎没有变化。细胞在交配前长时间的饥饿导致了同步性的逐渐丧失。
The time course and synchrony of the stages of conjugation in T. thermophila, as defined by cytologically observable changes in the morphology and position of the nuclei, were established. The time required for 50% of the pairs to enter or pass a particular stage and the duration of each stage were determined. The relative synchrony of the pairs as they went through conjugation was followed by correlating the maximum percentage of the population found in a stage with the duration of that stage. The degree of synchrony between the pairs was high under the conditions of this study, with very little decrease in synchrony seen during the initial 9 h of conjugation. Although some variability in the degree of synchrony was seen between different matings, there was little change detected in the duration of each cytological stage. Prolonged starvation of the cells prior to their mating resulted in a gradual loss of synchrony.