Simian varicella virus open reading frame 63/70 expression is required for efficient virus replication in culture
Simian varicella virus open reading frame 63/70 expression is required for efficient virus replication in culture
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DOI：
10.1007/s13365-011-0025-6
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发表时间：
2011-06-01
影响因子：
3.2
通讯作者：
Mahalingam, Ravi
中科院分区：
文献类型：
--
作者：
Brazeau, Elizabeth;Wellish, Mary;Mahalingam, Ravi
猴水痘病毒（SVV）开放阅读框（ORF）63在病毒基因组中复制为ORF 70，与水痘带状疱疹病毒ORF 63/70同源。将含有野生型SVV基因组和在ORF 70、ORF 63和70中具有终止密码子的突变体以及修复的病毒DNA序列的细菌人工染色体克隆转染到Vero细胞中产生细胞病变效应（CPE）。双突变体转染子的CPE发生慢得多（10天对3天），并且噬斑较小。虽然SVV ORF 63不是在培养物中复制所必需的，但其表达导致稳健的病毒复制。
Simian varicella virus (SVV) open reading frame (ORF) 63, duplicated in the virus genome as ORF 70, is homologous to varicella zoster virus ORF 63/70. Transfection of bacterial artificial chromosome clones containing the wild-type SVV genome and mutants with stop codons in ORF 70, in both ORFs 63 and 70 and the repaired virus DNA sequences into Vero cells produced a cytopathic effect (CPE). The onset of CPE was much slower with the double-mutant transfectants (10 days vs. 3 days) and plaques were smaller. While SVV ORF 63 is not required for replication in culture, its expression leads to robust virus replication.