Autoradiographic localization of specifically bound 125I-labeled follicle-stimulating hormone on spermatogonia of the rat testis.
Autoradiographic localization of specifically bound 125I-labeled follicle-stimulating hormone on spermatogonia of the rat testis.
复制标题
大鼠睾丸精原细胞上特异性结合 125I 标记的卵泡刺激素的放射自显影定位。
作者：
Joanne M. Orth;Christensen Ak
在以前的研究中，我们使用放射自显影术来证明大鼠曲细精管基底隔室Sertoli质膜上的特异性FSH结合位点。本研究旨在确定大鼠睾丸精原细胞是否也具有FSH结合位点。将约1 μ g [125 I]hFSH以睾丸内注射方式给予10天前切除垂体的40日龄大鼠;第二组大鼠接受500倍过量的冷激素沿着[125 I]- hFSH，并作为结合特异性的对照。随后，所有睾丸灌注高渗固定剂，产生优先收缩的精原细胞和支持细胞在基底室。由此产生的细胞彼此分离使我们能够确定精原细胞是否是任何放射自显影颗粒的来源。在缺乏过量冷激素的情况下，一些颗粒明显定位于精原细胞表面，但大部分颗粒位于精原细胞表面。
In a previous study, we used autoradiography to demonstrate specific FSH-binding sites on Sertoli plasma membranes in the basal compartment of rat seminiferous tubules. The present study was undertaken to determine whether spermatogonia in the rat testis also possess FSH-binding sites. Approximately 1 Mg [l25I]hFSH was administered as an intratesticular injection to 40-day-old rats that had been hypophysectomized 10 days earlier; a second group of rats received a 500-fold excess of cold hormone along with the [I25I]- hFSH, and served as a control for specificity of binding. Subsequently, all testes were perfused with a hyperosmotic fixative, which produces preferential shrinkage of spermatogonia and Sertoli cells in the basal compartment. The resulting separation of the cells from one another allowed us to determine whether spermatogonia were the source of any autoradiographic grains. In the absence of excess cold hormone, some grains were clearly localized to spermatogonial surfaces, although the majorit...