Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores
Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores
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DOI：
10.1128/mcb.18.11.6826
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发表时间：
1998-11-01
影响因子：
5.3
通讯作者：
Hurt, E
中科院分区：
文献类型：
--
作者：
Santos-Rosa, H;Moreno, H;Hurt, E
我们在Mex67p和Mtr2p之间发现了一个复合物，它对mRNA的输出至关重要。这种复合物，无论是从酵母中分离出来的还是在大肠杆菌中组装的，都可以通过Mex67p体外结合RNA。在体内，Mex67p需要Mtr2p才能与核孔结合，这可以通过突变MEX67或MTR2来消除。在所有情况下，Mex67p从孔中脱离进入细胞质与mRNA输出的强烈抑制相关。在核孔处，Nup85p是与Mex67p-Mtr2p复合物相互作用的靶标之一。因此，Mex67p和Mtr2p构成了一个新的mRNA输出复合体，它可以通过Mex67p与RNA结合，并通过Mtr2p与核孔相互作用。
We have identified between Mex67p and Mtr2p a complex which is essential for mRNA export. This complex, either isolated from yeast or assembled in Escherichia coli, can bind in vitro to RNA through Mex67p. In vivo, Mex67p requires Mtr2p for association with the nuclear pores, which can be abolished by mutating either MEX67 or MTR2. In all cases, detachment of Mex67p from the pores into the cytoplasm correlates with a strong inhibition of mRNA export. At the nuclear pores, Nup85p represents one of the targets with which the Mex67p-Mtr2p complex interacts. Thus, Mex67p and Mtr2p constitute a novel mRNA export complex which can bind to RNA via Mex67p and which interacts with nuclear pores via Mtr2p.