Monoclonal antibodies to surface antigens of rabbit type II pneumocytes.
Monoclonal antibodies to surface antigens of rabbit type II pneumocytes.
复制标题
兔 II 型肺细胞表面抗原的单克隆抗体。
DOI：
10.1164/arrd.1984.130.3.505
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Shapiro,DL
中科院分区：
文献类型：
--
作者：
Fraser,CM;Venter,JC;Finkelstein,JN;Shapiro,DL
报告了用于生产针对II型肺细胞的细胞表面抗原的单克隆抗体的技术。使用这些技术，从8只小鼠的脾细胞融合产生了200多个杂交瘤。其中，25个表达了对II型肺细胞表面的活性。许多抗体与来自其他器官和物种的各种细胞和膜交叉反应，表明这些抗体对膜抗原是普遍存在的。大多数抗体与犬肺膜发生强烈交叉反应，表明存在常见的哺乳动物肺决定簇。五个杂交瘤产生的上清液抗体具有相当大的特异性II型肺细胞。用腹水抗体检测5株杂交瘤细胞。II型肺细胞表面的免疫荧光染色，可以证明与所有5个这些抗体。本初步研究证明了生产针对II型肺细胞表面抗原的单克隆抗体的可行性。
Techniques for the production of monoclonal antibodies to cell surface antigens of type II pneumocytes are reported. Using these techniques, over 200 hybridomas were produced from spleen cell fusions of 8 mice. Of these, 25 expressed activity toward the type II pneumocyte cell surface. Many antibodies cross-reacted with a variety of cells and membranes from other organs and species, suggesting that these antibodies were ubiquitous to membrane antigens. Most of the antibodies cross-reacted strongly with dog lung membranes, suggesting the existence of common mammalian lung determinants. Five hybridomas produced supernatant antibody with considerable specificity for type II pneumocytes. Ascites fluid antibody was produced to these 5 hybridomas. Immunofluorescent staining of type II pneumocyte cell surfaces could be demonstrated with all 5 of these antibodies. This initial study demonstrates the feasibility of producing monoclonal antibodies to cell surface antigens of the type II pneumocyte.