Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis
Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis
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DOI：
10.1055/s-2008-1037541
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发表时间：
2008
影响因子：
1.3
通讯作者：
B. Straub;P. Stöffel;H. Heid;R. Zimbelmann;P. Schirmacher
中科院分区：
文献类型：
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作者：
B. Straub;P. Stöffel;H. Heid;R. Zimbelmann;P. Schirmacher
脂肪变性（脂肪变性）是西方国家最常见的肝脏病理学，并且由广泛的疾病如酒精滥用和代谢综合征引起。脂滴（LD）的表面层含有共享同源序列和结构域的蛋白质家族成员，即所谓的PAT蛋白，以其成分perilipin，adipophilin和TIP47命名。我们表征了正常和患病肝脏中与LD蓄积相关的LD相关蛋白。Adipophilin和TIP47在储存维生素A的肝星状细胞的LD中以及另外在脂肪变性肝细胞的LD中表达。脂周蛋白被认为是脂肪细胞和类固醇生成细胞LD的特征，在人脂肪变性肝的肝细胞中重新表达。通过生物化学、分子生物学和免疫组织化学方法，在人脂肪肝细胞中发现了周脂蛋白剪接变异体A。它与LD的关联不同于TIP47和亲脂蛋白，并且取决于LD的大小和定位，这表明不同的PAT蛋白在LD的成熟过程中发挥特定的作用。（肝脏学2008年）
Fatty change (steatosis) is the most frequent liver pathology in western countries and is caused by a broad range of disorders such as alcohol abuse and metabolic syndrome. The surface layer of lipid droplets (LDs) contains members of a protein family that share homologous sequences and domains, the so‐called PAT proteins, named after their constituents, perilipin, adipophilin, and TIP47. We characterized the LD‐associated proteins in normal and diseased liver connected with LD accumulation. Adipophilin and TIP47 are expressed in LDs of vitamin A‐storing hepatic stellate cells and additionally in LDs of steatotic hepatocytes. Perilipin, which was thought to be characteristic for LDs of adipocytes and steroidogenic cells, becomesde novoexpressed in hepatocytes of human steatotic liver. Perilipin splice variant A was found in human steatotic hepatocytes by biochemical, molecular biological, and immunohistochemical methods. Its association with LDs is different from TIP47 and adipophilin, and depends on size and localization of the LDs, suggesting that the different PAT proteins play specific roles during maturation of LDs. (HEPATOLOGY2008.)