RELATION BETWEEN PHOSPHATE METABOLITES AND OXYGEN-CONSUMPTION OF HEART INVIVO
RELATION BETWEEN PHOSPHATE METABOLITES AND OXYGEN-CONSUMPTION OF HEART INVIVO
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DOI：
10.1152/ajpheart.1989.256.1.h265
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发表时间：
1989-01-01
影响因子：
--
通讯作者：
BALABAN, RS
中科院分区：
文献类型：
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作者：
KATZ, LA;SWAIN, JA;BALABAN, RS
在犬体内心脏中研究了诱导增加的心脏工作和磷酸盐代谢产物之间的关系，以评估ATP水解产物ADP和无机磷酸盐（Pi）在控制心肌耗氧量（M.ovrhdot.VO2）中的作用。在这些研究中，心肌血流量和耗氧量与31 P-核磁共振（NMR）-检测磷酸盐代谢产物同时测量。三种方案用于增加心肌作功：起搏、肾上腺素和苯肾上腺素输注。当这些协议被使用，没有或只有轻微的变化，心肌ATP，Pi，磷酸肌酸观察到大于三倍的增加M.ovrhdot.VO2。计算的细胞内游离镁浓度，ADP，和pH值也只有轻微的影响，这些增加的工作。这些数据表明，一个简单的模型，涉及反馈的胞质ADP和Pi的线粒体调节呼吸是不足以解释呼吸控制在体内。这些数据表明，其他一些参数或协同效应，涉及磷酸盐代谢产物必须发挥作用，在体内心脏呼吸和工作之间的反馈。
The relation between induced increases in cardiac work and phosphate metabolites was investigated in the canine heart in vivo to evaluate the role of ATP hydrolysis products, ADP and inorganic phosphate (Pi), in the control of myocardial oxygen consumption (M.ovrhdot.VO2). In these studies, myocardial blood flow and oxygen consumption were simultaneously measured with the 31P-nuclear magnetic resonance (NMR)-detected phosphate metabolites. Three protocols were used to increase myocardial work:pacing, epinephrine, and phenylephrine infusions. When these protocols were used, no or only slight changes in myocardial ATP, Pi, and creatine phosphate were observed with a greater than threefold increase in M.ovrhdot.VO2. The calculated intracellular free Mg concentration, ADP, and pH were also only slightly affected by these increases in work. These data indicate that a simple model involving the feedback of cytosolic ADP and Pi to the mitochondria regulating respiration is inadequate to explain respiratory control in vivo. These data suggest that some other parameters or cooperativity effects involving the phosphate metabolites must play a role in the feedback between respiration and work in the heart in vivo.