Optimisation of poly-β-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems
Optimisation of poly-β-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems
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DOI：
10.1016/j.chroma.2005.02.020
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发表时间：
2005-04-08
影响因子：
4.1
通讯作者：
Keller, E
中科院分区：
文献类型：
--
作者：
Oehmen, A;Keller-Lehmann, B;Keller, E
聚-β-羟基链烷酸酯（PHA）是一种常用于许多不同细菌细胞碳和能量储存的聚合物。聚磷菌（PAOs）和糖原累积菌（GAOs）通过乙酸盐和丙酸盐等碳底物的代谢在厌氧条件下储存PHA。尽管聚-β-羟基丁酸酯（PHB）和聚-β-羟基戊酸酯（PHV）通常使用先前开发的气相色谱（GC）方法进行定量，但聚-β-羟基-2 - 甲基戊酸酯（PH2MV）却很少被定量，尽管已有研究表明它是PAOs或GAOs代谢丙酸盐时产生的一种关键的PHA组分。本文介绍了两种基于气相色谱的方法，这些方法经过改进可用于从强化生物除磷（EBPR）系统中提取和定量PHB、PHV和PH2MV。对于从以乙酸盐为底物的PAO和GAO培养物中提取PHB和PHV，建议使用3%的硫酸浓度和2 - 20小时的消解时间，而对于从以丙酸盐为底物的EBPR系统中进行PHV和PH2MV分析，建议使用10%的硫酸溶液消解20小时。（c）2005爱思唯尔有限公司。保留所有权利。
Poly-beta-hydroxyalkanoate (PHA) is a polymer commonly used in carbon and energy storage for many different bacterial cells. Polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs), store PHA anaerobically through metabolism of carbon substrates such as acetate and propionate. Although poly-beta-hydroxybutyrate (PHB)and poly-beta-hydroxyvalerate (PHV) are commonly quantified using a previously developed gas chromatography (GC) method, poly-beta-hydroxy-2-methyl valerate (PH2MV) is seldom quantified despite the fact that it has been shown to be a key PHA fraction produced when PAOs or GAOs metabolise propionate. This paper presents two GC-based methods modified for extraction and quantification of PHB, PHV and PH2MV from enhanced biological phosphorus removal (EBPR) systems. For the extraction Of PHB and PHV from acetate fed PAO and GAO cultures, a 3% sulfuric acid concentration and a 2-20 h digestion time is recommended, while a 10% sulfuric acid solution digested for 20 h is recommended for PHV and PH2MV analysis from propionate fed EBPR systems. (c) 2005 Elsevier B.V. All rights reserved.