Lipid absorption in different segments of the gastrointestinal tract of Atlantic salmon (Salmo salar L.)
Lipid absorption in different segments of the gastrointestinal tract of Atlantic salmon (Salmo salar L.)
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DOI：
10.1016/j.aquaculture.2004.06.030
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发表时间：
2004-10-27
期刊：
影响因子：
4.5
通讯作者：
Ruyter, B
中科院分区：
文献类型：
--
作者：
Denstadli, V;Vegusdal, A;Ruyter, B
研究了大西洋鲑鱼在强制饲喂标准养殖者饮食后的胃肠道脂质吸收情况，其中添加了同位素标记的游离脂肪酸 (FFA) [1-C-14]-癸酸 (C-14-10:0) 和三酰甘油 (TAG) [9, 10-H-3(N)]-三油酸甘油酯（三油酸；H-3-18:1）。强行喂食后，将15条鱼平均体重704克，饲养在5个圆形水箱中，每个水箱3条，供给海水（盐度32g·l(-1)）。在第3、6、12、18和48小时，杀死一缸中的鱼，取出胃肠道（GI）并分为六段：胃（ST）、前幽门盲肠、中盲肠和远端盲肠（分别为PCA、PCM和PCD）、中肠（MI）和远端肠（DI）。在每个节段的组织样本中测量 C-14-10:0 和 H-3-18:1 底物的放射性水平（dpm [每分钟崩解数] mug(-1) 组织），从而可以估计从管腔吸收到肠细胞中的脂肪酸底物的量。强制喂食后 12 至 18 小时，在幽门盲肠 (PC) 组织中测量到两种脂肪酸底物的最高放射性水平。 PCA 中 C-14-10:0 底物的放射性水平显着高于 PCM 和 PCD，而不同 PC 组织片段中测量的 H-3-18:1 底物放射性水平与 MI 中测量的水平没有明显差异。对于两种脂肪酸底物来说，DI 造成的组织中的放射性水平可以忽略不计甚至很低。使用光学显微镜和图像分析在每个采样点对来自 PCM、MI 和 DI 的组织中的总脂质积累进行形态测定。这些结果证实了同位素研究的结果，即强制喂食后 12 小时和 18 小时内脂肪含量最高。总之，PC 是大西洋鲑鱼胃肠道中脂肪酸吸收最重要的部位。 (C) 2004 Elsevier B.V. 保留所有权利。
Gastrointestinal lipid absorption in Atlantic salmon was studied following force-feeding with a standard grower diet to which isotope-labelled free fatty acid (FFA) [1-C-14]-decanoic acid (C-14- 10:0) and triacylglycerol (TAG) [9, 10-H-3(N)]-triolein (trioleic acid; H-3-18:1) were added. Following force-feeding, the 15 fish, with an average weight of 704 g, were kept in five circular tanks, three fish per tank, supplied with seawater (salinity, 32 g l(-1)). At 3, 6, 12, 18 and 48 h, the fish from one tank were killed, and the gastrointestinal (GI) tract was removed and divided into six segments: stomach (ST), anterior, mid, and distal pyloric ceca (PCA, PCM, and PCD, respectively), mid intestine (MI), and distal intestine (DI). The levels of radioactivity (dpm [disintegrations per minute] mug(-1) tissue) from the C-14-10:0 and the H-3-18:1 substrates were measured in tissue samples from each segment, allowing an estimation of the amounts of fatty acid substrates absorbed from the lumen into the intestinal cells. The highest levels of radioactivity from both fatty acid substrates were measured in tissue from pyloric ceca (PC) 12 to 18 h after force-feeding. The level of radioactivity from the C-14-10:0 substrate was significantly higher in the PCA than in the PCM and PCD, while there was no clear difference in the level of radioactivity from the H-3-18:1 substrate measured among the different PC tissue segments and the level measured in MI. The level of radioactivity in tissue from DI was negligible to low for both fatty acid substrates. Total lipid accumulation in tissue from PCM, MI and DI was quantified morphometrically at each sampling point using light microscopy and image analysis. These results confirmed the findings from the isotope study, where the highest levels of fat were present at 12 and 18 h following force-feeding. In conclusion, PC was the most important site for fatty acid absorption in the GI tract of Atlantic salmon. (C) 2004 Elsevier B.V. All rights reserved.