Profiles of proinflammatory cytokines in sputum from different groups of severe asthmatic patients
Profiles of proinflammatory cytokines in sputum from different groups of severe asthmatic patients
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DOI：
10.1016/s1081-1206(10)60795-8
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发表时间：
2006-09-01
影响因子：
5.9
通讯作者：
Paggiaro, Pierluigi
中科院分区：
文献类型：
--
作者：
Dente, Federico L.;Carnevali, Stefano;Paggiaro, Pierluigi
背景：严重哮喘是一个异质性的患者群体，其气道炎症特征知之甚少。目的：评估不同表型严重哮喘的痰细胞因子谱。方法：将严重哮喘患者 (n = 45) 分为 3 组：频繁发作、持续性支气管收缩和两者兼有。另外两组（9 名未经治疗的轻度哮喘患者和 10 名对照受试者）也进行了研究。对选定的痰液部分进行分类细胞计数、上清白细胞介素 5 (IL-5)、粒细胞-巨噬细胞集落刺激因子、IL-8 和嗜酸性粒细胞阳离子蛋白的检测。结果：3 个严重哮喘组之间的痰液炎症细胞百分比、IL-8 水平和嗜酸性粒细胞阳离子蛋白水平没有统计学显着差异，尽管哮喘患者的 IL-8 水平往往较高。持续性支气管收缩。与其他两组相比，频繁发作的患者痰中粒细胞-巨噬细胞集落刺激因子和 IL-5 浓度显着升高。与轻度哮喘患者和对照组相比，重度哮喘患者的 IL-5 和 IL-8 水平较高，而痰液嗜酸性粒细胞百分比介于轻度哮喘患者和对照组之间。结论：频繁发作的重度哮喘患者中嗜酸性粒细胞细胞因子水平升高，但持续支气管收缩的重度哮喘患者中嗜酸性粒细胞细胞因子水平不升高，这表明不同的细胞因子谱可能与重度哮喘的不同表型相关。
Background: Severe asthma represents a heterogeneous group of patients whose characteristics of airway inflammation are poorly known.Objective: To evaluate the sputum cytokine profiles of different phenotypes of severe asthma.Methods: Severe asthmatic patients (n = 45) were divided into 3 groups: frequent exacerbations, persistent bronchoconstriction, and both features. Two other groups (9 patients with untreated mild asthma and 10 control subjects) were also studied. Selected sputum portions were assayed for differential cell count, supernatant interleukin 5 (IL-5), granulocyte-macrophage colony-stimulating factor, IL-8, and eosinophil cationic protein.Results: There were no statistically significant differences among the 3 severe asthma groups in terms of sputum inflammatory cell percentages, IL-8 levels, and eosinophil cationic protein levels, although IL-8 levels tended to be higher in patients with persistent bronchoconstriction. Sputum concentrations of granulocyte-macrophage colony-stimulating factor and IL-5 were significantly higher in patients with frequent exacerbations compared with the other 2 groups. Levels of IL-5 and IL-8 were higher in severe asthmatic patients compared with mild asthmatic patients and controls, whereas sputum eosinophil percentages were intermediate between those of mild asthmatic patients and controls.Conclusions: Proeosinophilic cytokine levels are increased in severe asthmatic patients with frequent exacerbations but not in severe asthmatic patients with persistent bronchoconstriction, suggesting that different cytokine profiles could be associated with different phenotypes of severe asthma.