High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives.
High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives.
复制标题
含有末端赖氨酸或鸟氨酸及其丹酰衍生物的甲氨蝶呤类似物的高效液相色谱法。
DOI：
10.1016/0003-2697(83)90362-7
复制
发表时间：
1983
影响因子：
2.9
通讯作者：
Freisheim,JH
中科院分区：
文献类型：
--
作者：
Kumar,AA;Kempton,RJ;Anstead,GM;Price,EM;Freisheim,JH
A procedure utilizing a reverse-phase semipreparative high-performance liquid chromatography column and a binary solvent system consisting of trifluoroacetic acid and 1-propanol has been developed for the semipreparative scale purification and analytical identification of four newly synthesized analogs of methotrexate. The methotrexate analogs containing a lysine or an ornithine residue in place of a terminal glutamate residue together with their respective dansyl derivatives were purified in milligram quantities by the procedures described.
