Effect of green light on the amount and activity of NDH-1-PSI supercomplex in Synechocystis sp strain PCC 6803
Effect of green light on the amount and activity of NDH-1-PSI supercomplex in Synechocystis sp strain PCC 6803
复制标题
绿光对集胞藻PCC 6803菌株NDH-1-PSI超复合物数量和活性的影响
DOI：
10.1007/s11099-018-0790-z
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发表时间：
2018
期刊：
影响因子：
2.7
通讯作者：
Ma Weimin
中科院分区：
文献类型：
--
作者：
Gao Fudan;Ogawa Teruo;Ma Weimin
蓝藻NDH-1与PSI相互作用形成NDH-1-PSI超复合物。CpcG 2是PSI特异性外周天线CpcG 2-藻胆体的连接蛋白，对于超复合物的稳定是必不可少的。与红光（RL）下这些组分的丰度相比，绿色光（GL）增加CpcG 2的表达，但对NDH-1和PSI的表达几乎没有影响（如果有的话）。CpcG 2表达的增加增强了类囊体膜蓝色天然凝胶电泳后NDH-1-PSI超复合物的条带，可能是通过稳定超复合物。当RL下生长的细胞转移到低强度GL时，PSI周围的NDH-1依赖性循环电子传递的活性增加，但当细胞在高强度GL下生长时，其活性受到抑制。PSI的功能性表现出相同的趋势。因此，我们得出结论，GL增加CpcG 2的表达，从而增加NDH-1-PSI超复合物的丰度及其在低GL下的活性，但在高GL下不增加。
Cyanobacterial NDH-1 interacts with PSI to form NDH-1–PSI supercomplex. CpcG2, a linker protein for the PSI-specific peripheral antenna CpcG2-phycobilisome, is essential for stabilization of the supercomplex. Green light (GL) increased the expression of CpcG2 but had little effect, if any, on the expression of NDH-1 and PSI, when compared to the abundance of these components under red light (RL). The increased expression of CpcG2 intensified the band of NDH-1–PSI supercomplex after blue-native gel electrophoresis of the thylakoid membrane, possibly by stabilizing the supercomplex. The activity of NDH-1-dependent cyclic electron transport around PSI increased when cells grown under RL were transferred to a low intensity GL but was suppressed when cells were grown under high intensities of GL. The functionality of PSI showed the same trend. We thus conclude that GL increases the expression of CpcG2, thereby increasing the abundance of the NDH-1–PSI supercomplex and its activity at low GL but not at higher GL.