Saturated hydrogen improves lipid metabolism disorders and dysbacteriosis induced by a high-fat diet
Saturated hydrogen improves lipid metabolism disorders and dysbacteriosis induced by a high-fat diet
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饱和氢可改善高脂饮食引起的脂质代谢紊乱和菌群失调
DOI：
10.1177/1535370219898407
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发表时间：
2020-01-07
影响因子：
3.2
通讯作者：
Wu, Guojun
中科院分区：
文献类型：
--
作者：
Qiu, Xiangjie;Ye, Qiaona;Wu, Guojun
研究表明，肥胖等代谢性疾病与肠道植物群失衡显著相关。乙醛酸循环引起的条件致病菌的扩增导致肠道植物群失衡。然而，有希望的是，饱和氢可以有效地改善代谢疾病的发生和发展，如肥胖。然而，饱和氢如何运作的具体机制仍然不是很清楚。在本研究中，高脂饮食后，小鼠外周血中的总胆固醇、总甘油酯和低密度脂蛋白水平升高，而高密度脂蛋白水平降低。肠脂肪酸代谢相关基因载脂蛋白E（ApoE）、脂肪酸合成酶（FAS）、肠脂肪酸结合蛋白（I-FAPB）、乙酰辅酶A羧化酶1（ACC 1）、过氧化物酶体增殖物激活受体γ（PPARγ）和硬脂酰辅酶A去饱和酶1（SCD 1）显著增加。粪便中的拟杆菌、双歧杆菌和乳酸杆菌计数显著下降，而阴沟肠杆菌增加。粪便中异柠檬酸裂解酶活性明显升高。用饱和氢处理小鼠导致外周血中总胆固醇、总甘油酯和低密度脂蛋白降低，高密度脂蛋白增加。小肠FAS和I-FAPB基因表达降低。粪便中的拟杆菌、双歧杆菌和乳酸杆菌显著增加，而阴沟肠杆菌减少。异柠檬酸裂解酶活性也明显降低。说明饱和氢可通过抑制肠道植物群的乙醛酸循环，改善肠道结构完整性和脂质代谢紊乱。过去的研究表明，氢气可以改善代谢紊乱，但其作用机制仍不清楚。众所周知，代谢性疾病如肥胖症与肠道植物群的变化显著相关。乙醛酸循环是原核生物、低等真核生物和植物的重要代谢途径，可能是代谢紊乱相关机制的门户。许多机会致病菌可以利用乙醛酸循环回收脂肪酸以合成糖和其他致病物质。因此，乙醛酸循环可能参与了高脂饮食条件下肠道菌群失调。因此，这项研究旨在提供氢如何改善代谢疾病的机制，并为氢在代谢疾病治疗中的应用提供新的依据。
Studies have shown that metabolic diseases, such as obesity, are significantly associated with intestinal flora imbalance. The amplification of opportunistic pathogens induced by the glyoxylic acid cycle contributes to intestinal flora imbalance. Promising, though, is that saturated hydrogen can effectively improve the occurrence and development of metabolic diseases, such as obesity. However, the specific mechanism of how saturated hydrogen operates is still not very clear. In this study, after a high-fat diet, the level of total cholesterol, total glyceride, and low-density lipoprotein in the peripheral blood of mice increased, and that of high-density lipoprotein decreased. Intestinal fatty acid metabolism-related gene Apolipoprotein E (ApoE), fatty acid synthase (FAS), intestinal fatty acid-binding protein (I-FAPB), acetyl-CoA carboxylase 1 (ACC1), peroxisome proliferator-activated receptor γ (PPARγ), and stearoyl-CoA desaturase 1 (SCD1) increased significantly. Bacteroides, Bifidobacteria, and Lactobacillus counts in feces decreased considerably, while Enterobacter cloacae increased. The activity of isocitrate lyase in feces increased markedly. Treatment of mice with saturated hydrogen led to decreased total cholesterol, total glyceride, and low-density lipoprotein and increased high-density lipoprotein in the peripheral blood. FAS and I-FAPB gene expression in the small intestine decreased. Bacteroides, Bifidobacteria, and Lactobacillus in feces increased significantly, whereas Enterobacter cloacae decreased. The activity of isocitrate lyase also diminished remarkably. These results suggest that saturated hydrogen could improve intestinal structural integrity and lipid metabolism disorders by inhibiting the glyoxylic acid cycle of the intestinal flora. Impact statement Past studies have shown that hydrogen can improve metabolic disorders, but its mechanism of action remains unclear. It is well known that metabolic diseases, such as obesity, are significantly associated with changes in the intestinal flora. The glyoxylic acid cycle is an essential metabolic pathway in prokaryotes, lower eukaryotes, and plants and could be the portal for mechanisms related to metabolic disorders. Many opportunistic pathogenic bacteria can recycle fatty acids to synthesize sugars and other pathogenic substances using the glyoxylic acid cycle. So, the glyoxylic acid cycle may be involved in intestinal dysbacteriosis under high-fat diet. This study, therefore, seeks to provide the mechanism of how hydrogen improves metabolic diseases and a new basis for the use of hydrogen in the treatment of metabolic disorders.