Association and colocalization of G protein alpha subunits and Purkinje cell protein 2 (Pcp2) in mammalian cerebellum.
Association and colocalization of G protein alpha subunits and Purkinje cell protein 2 (Pcp2) in mammalian cerebellum.
复制标题
哺乳动物小脑中 G 蛋白 α 亚基和浦肯野细胞蛋白 2 (Pcp2) 的关联和共定位。
DOI：
10.1002/jnr.10460
复制
发表时间：
2002
影响因子：
4.2
通讯作者：
Luo,Yuan
中科院分区：
文献类型：
--
作者：
Redd,KacyJ;Oberdick,John;McCoy,John;Denker,BradleyM;Luo,Yuan
Previously, we have demonstrated a novel interaction between Gαoprotein and Purkinje cell protein‐2 (Pcp2, also known as L7) in vitro and in transfected cells (Luo and Denker [1999] J. Biol. Chem. 274:10685–10688). Pcp2 is uniquely expressed in cerebellar Purkinje cells and in retinal bipolar neurons, and it may function as a cell‐type specific modulator for G protein‐mediated cell signaling. This interaction has been further evaluated in the present studies. Coimmunoprecipitation experiments reveal that Pcp2 associates with Gαoin vivo in mouse cerebellum and eye extract. Pcp2 also associate with Gαi2in the cerebellum. No detectable associations of Pcp2 with Gαzand Gαqsubunits are observed. The association of Gαoand Pcp2 is detected at postnatal day 1 (P1), and the association remains stable from day 3 (P3) until adulthood. Further, immunofluorescent double labeling and confocal microscopy suggest that Pcp2 and Gαoare colocalized in the distal processes of cerebellar Purkinje cells including axonal endings and dendritic spines. Taken together, these findings indicate colocalization and association of Gαoand Pcp2 in cerebellum and suggest a functional role in regions of synaptic activity. © 2002 Wiley‐Liss, Inc.
