Label-free STORM principle realized by super-Rayleigh speckle in photoacoustic imaging.
Label-free STORM principle realized by super-Rayleigh speckle in photoacoustic imaging.
复制标题
光声成像中超瑞利散斑实现的无标记STORM原理。
作者：
Penghuan Liu
光声成像可以提供隐藏在生物组织内部的信息，但其分辨率受到声衍射的限制。它已被证明，不受控制的散斑照明，这是已知的盲结构照明光声显微镜（BSIPAM），可以帮助超过这个分辨率障碍。虽然超分辨能力已经在合成和实验数据上得到了验证，但这种系统所能达到的理论分辨极限仍然不清楚。这封信表明，PA成像系统中的随机光学重建显微镜（STORM）的原理可以通过定制的超瑞利散斑来实现;因此，可以预期优于两倍的超分辨率。
Photoacoustic (PA) imaging can provide information hidden deep inside biological tissues; however, its resolution is limited by acoustic diffraction. It has been demonstrated that the uncontrolled speckle illumination, which is known as blind structured illumination photoacoustic microscopy (BSIPAM), can help surpass this resolution barrier. Although the super-resolution capacity has been verified on both synthesized and experimental data, the achievable theoretical resolution limit by such a system is still unclear. This Letter shows that the principle of Stochastic Optical Reconstruction Microscopy (STORM) in a PA imaging system can be implemented by the tailored super-Rayleigh speckle; thus, a super-resolution better than a factor of two can be expected.
DOI：
10.1073/pnas.0406877102
发表时间：
2005-09-13
影响因子：
11.1
作者：
Gustafsson, MGL
通讯作者：
Gustafsson, MGL