Comparison of serum and urine assays for biochemical markers of bone resorption in postmenopausal women with and without hormone replacement therapy and in men
Comparison of serum and urine assays for biochemical markers of bone resorption in postmenopausal women with and without hormone replacement therapy and in men
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DOI：
10.1007/s001980070089
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发表时间：
2000-01-01
影响因子：
4
通讯作者：
Raisz, LG
中科院分区：
文献类型：
--
作者：
Fall, PM;Kennedy, D;Raisz, LG
骨吸收的生化标记物已被用于确定代谢性骨病的特征和评估治疗反应。大多数研究使用尿胶原交联物的测量，但最近开发的血清分析可能具有较少的分析和生物变异性。在本研究中，我们检测了接受或不接受激素替代治疗(HRT)的绝经后妇女和同龄男性的尿液和血清I型胶原N-端和C-端交联末端肽(NTX和CTX)和血清骨唾液蛋白(BSP)。在这些人群中，血清和尿液标记物的变异性相似，除了绝经后妇女血清NTX的变异性较低。接受激素替代治疗的绝经后妇女的尿液和血清检测结果相互之间有很好的相关性，并且显著低于未经治疗的妇女。在接受WRT的女性中，sNTX、uNTX和BSP的差异相似(35%-40%)，而sCTX和uCTX的差异更大(52%-53%)。标记物与骨密度之间呈负相关，这主要归因于未接受IRT的女性的高度相关性。NTX和CTX的排泄分数分别为0.20+/-0.07和0.34+/-0.11。这些值与标记物或尿中肌酐的浓度无关。我们的结论是，血清标记物是这些人群中骨RT吸收的有用指标，在这些人群中，使用这些标记物可能有助于骨质疏松的治疗。
Biochemical markers of bone resorption have been used to characterize metabolic bone disease and assess therapeutic response. Most studies have used the urinary measurement of collagen crosslinks, but serum assays have recently been developed that may have less analytic and biologic variability. In the present study, we measured urine and serum N- and C-terminal crosslinked telopeptides of typo I collagen (NTX and CTX) and serum bone sialoprotein (BSP) in postmenopausal women with or without hormone replacement therapy (HRT) and in men of similar age. In these populations, the variability of serum and urine markers was similar, except that serum NTX showed somewhat lower variability in postmenopausal women. Urine and serum assays correlated well with one another and were significantly lower in postmenopausal women on HRT compared with untreated women. The difference in women on WRT was similar for sNTX, uNTX and BSP (35-40%) and greater for sCTX and uCTX (52-53%). There was an inverse correlation between markers and bone mineral density, largely attributable to the high correlation in women not on I IRT. Fractional excretion of NTX and CTX were estimated at 0.20 +/- 0.07 and 0.34 +/- 0.11, respectively. These values were independent of the concentration of the marker or of creatinine in the urine. We conclude that serum markers are useful measures of bone rt sorption in these populations, in whom the use of such markers is likely to be helpful in the management of osteoporosis.