Assessing the risk of skin damage due to femtosecond laser irradiation
Assessing the risk of skin damage due to femtosecond laser irradiation
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DOI：
10.1002/jbio.200810050
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发表时间：
2008-12-01
影响因子：
2.8
通讯作者：
Wepf, Roger
中科院分区：
文献类型：
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作者：
Fischer, Frank;Volkiner, Beate;Wepf, Roger
我们用四种通常用于多光子成像的照射方案照射新鲜切除的皮肤活检。如果有任何皮肤损伤，它主要是一种类似于紫外线照射的损伤作用。使用针对环丁烷-嘧啶-二聚体（CPD）的荧光抗体结合免疫荧光图像分析，我们定量地比较了FS-辐射与UV-辐射（太阳模拟器）的影响。根据这些结果，我们正在进行风险评估。结果表明，使用这里描述的参数的多光子成像是在每天的阳光照射所发生的损伤的棒球场。人表皮染色的抗体对环丁烷-嘧啶-二聚体（CPD）。在用CPD抗体进行荧光染色之前，使用高功率超短脉冲聚焦NIR激光照射[GRAPHICS]（飞秒激光照射）照射基底膜周围的离体皮肤样品。激光功率设置得比通常用于成像的高得多（在毫瓦范围内）。CPD的构建是由于这种高功率激光照射最有可能在表皮中引入UV样损伤效应。(c)2008年由WILEY-VCH Verlag GmbH & Co. KGaA，魏因海姆
We irradiated freshly excised skin biopsies with four irradiation regimes usually taken for multiphoton imaging. If there is any skin damaging, it is mainly an effect similar to the damaging effects of UV-irradiation. Using fluorescent antibodies against cyclobutane-pyrimidin-dimers (CPDs) in combination with immuno-fluorescence image analysis we quantitatively compared fs-irradiation effects with UV-irradiation (solar simulator). Based on these results we are giving a risk assessment. The results show that multi photon imaging using the parameters described here is in the ballpark of damaging occurring from every day sun exposure.Human epidermis stained with an antibody against cyclobutane-pyrimidin-dimer (CPD). Prior to fluorescence staining with CPD antibodies the ex vivo skin sample was irradiated around the basal membrane using high power ultra short, pulsed focused NIR laser irradiation[GRAPHICS](femtosecond laser irradiation). The laser power was set much higher than usually used for imaging (in the milliwatt-range). The CPDs were built due to this high power laser irradiation most likely introducing an UV-like damaging effect in the epidermis. (c) 2008 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim