Recent advances in our knowledge of Mycobacterium bovis:: A feeling for the organism
Recent advances in our knowledge of Mycobacterium bovis:: A feeling for the organism
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DOI：
10.1016/j.vetmic.2005.11.050
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发表时间：
2006-02-25
影响因子：
3.3
通讯作者：
Gordon, SV
中科院分区：
文献类型：
--
作者：
Hewinson, RG;Vordermeier, HM;Gordon, SV
自上一届国际牛分枝杆菌大会以来，我国对牛分枝杆菌的认识和了解取得了重大而迅速的进展。五年前的Bovis会议。这一进展的大部分都得到了基因组测序完成的支持。这一重要的里程碑促进了对开发一些用于防治牛结核病的改进工具的研究。在这篇文章中，我们将回顾最近取得的进展，在这些工具的发展，并在我们的有机体，其进化和传播的理解。将该基因组序列与结核分枝杆菌复合体其他成员的基因组序列进行比较，有助于深入了解结核分枝杆菌的进化。牛这一分析还表明，M。结核复合体具有适应新宿主物种的倾向。高通量分子分型方法的使用表明，最近在英国的牛结核病流行是由许多克隆扩增驱动的，这不能仅用随机突变和漂移来解释。许多分支杆菌基因组序列的完成使得快速识别分支杆菌的抗原挖掘技术得以开发。牛的特异性基因然后，这些可用作γ干扰素测定中的试剂，以增加测定的特异性，并区分接种卡介苗（BCG）的动物和感染支原体的动物。牛从长远来看，M.牛和BCG的研究将使我们能够深入了解BCG如何在连续传代油人工生长培养基后减毒，并揭示如何提高BCG疫苗效力的线索。皇冠版权所有（c）2005年出版的爱思唯尔B.V.保留所有权利。
Significant and rapid progress has been made in Our knowledge and understanding of Mycobacterium bovis since the last international M. bovis conference 5 years ago. Much of this progress has been underpinned by the completion of the genome sequence. This important milestone has catalysed research into the development of a number of improved tools with which to combat bovine tuberculosis. In this article we will review recent progress made in the development of these tools and in our understanding of the organism, its evolution and spread. Comparison of the genome sequence with those of other members of the Mycobacterium tuberculosis complex has enabled insights into the evolution of M. bovis. This analysis also indicates that the M. tuberculosis complex have the propensity to adapt to new host species. The use of high throughput molecular typing methods has revealed that the recent bovine tuberculosis epidemic in Great Britain is being driven by a number of clonal expansions, which cannot be explained by random Mutation and drift alone. Completion of a number of mycobacterial genome sequences has allowed the development of antigen mining techniques that rapidly identify M. bovis-specific genes. These can then be used as reagents in the gamma interferon assay to increase the specificity of the assay and also to discriminate between Bacillus of Calmette and Guerin (BCG) vaccinated animals and those infected with M. bovis. In the longer term, comparisons between the genomes of M. bovis and BCG will allow insight into how BCG became attenuated following serial passage oil artificial growth media and reveal clues into how to improve the vaccine efficacy of BCG. Crown Copyright (c) 2005 Published by Elsevier B.V. All rights reserved.