COLCHICINE-INDUCED POLYPLOIDY IN CITRUS
COLCHICINE-INDUCED POLYPLOIDY IN CITRUS
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DOI：
10.1086/336726
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发表时间：
1974-01-01
影响因子：
--
通讯作者：
BARRETT, HC
中科院分区：
文献类型：
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作者：
BARRETT, HC
用秋水仙素处理20个柑桔属种及其杂种的二倍体无性系和1个柑桔属的二倍体无性系，获得供育种试验用的多倍体。详细介绍了用于植物制备，秋水仙素处理，鉴定和分离多倍体组织，和用于识别产生的细胞嵌合体的显微技术。发现在宏观筛选中有用的诊断特征是扇形图案的叶、叶颜色变化、叶柄叶片形状、加厚的叶片脉序、油腺和气孔的比较大小差异、叶片厚度和叶形状。八种类型的细胞嵌合体从秋水仙素影响的材料的细胞学检查确定。数据给出的品种多倍化秋水仙素，类型的组织发生层变化分离每个克隆，频率分离每个克隆，和某些叶的测量和关系。数据还提出了层的变化和分布的数量和频率和频率的类型的细胞嵌合体在多胚和单胚克隆。结果表明，在多倍体的诱导和表达，秋水仙素的差异反应存在于克隆的遗传组成，在个别组织发生层，并在存在或不存在的不定胚的频率的细胞类型和层协会进行了讨论，在可能的起源模式。讨论了与无性系内变异和柑橘育种有关的层间关联和起源方面，并提到了柑橘细胞嵌合体的一些潜在用途。
Twenty diploid clones of Citrus species and hybrids and one diploid clone of Fortunella were treated with colchicine to develop polyploids for use in breeding experiments. Details on the techniques used for plant preparation, colchicine treatment, identification and isolation of polyploid tissue, and the microtechnique used to identify the cytochimeras produced are described. Diagnostic features found to be useful in macroscopic screening were sectorially patterned leaves, leaf color changes, petiole blade shape, thickened leaf venation, comparative size differences in oil glands and stomates, leaf blade thickness, and leaf shape. Eight types of cytochimeras were identified from cytological examination of colchicine-affected material. Data are given for cultivars polyploidized by colchicine, type of histogenic layer change isolated per clone, frequency of isolations per clone, and certain leaf measurements and relationships. Data are also presented for the number and frequency of layer changes and distribution and frequency of types of cytochimeras in polyembryonic and monoembryonic clones. Results demonstrate that in the induction and expression of polyploidy, a differential response to colchicine exists in the genetic constitution of clones, in the individual histogenic layers, and in the presence or absence of adventitious embryony The frequency of cytological types and layer associations is discussed in relation to possible modes of origin. Aspects of layer association and origin in relation to intraclonal variation and citrus breeding are discussed Some potential uses of citrus cytochimeras are mentioned.