Functional integration of the HUP1 hexose symporter gene into the genome of C-reinhardtii:: Impacts on biological H2 production
Functional integration of the HUP1 hexose symporter gene into the genome of C-reinhardtii:: Impacts on biological H2 production
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DOI：
10.1016/j.jbiotec.2007.05.017
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发表时间：
2007-08-01
影响因子：
4.1
通讯作者：
Kruse, Olaf
中科院分区：
文献类型：
--
作者：
Doebbe, Anja;Rupprecht, Jens;Kruse, Olaf
光养生物利用光合作用将太阳能转化为化学能。在自然界中，化学能储存在各种生物聚合物中。这些来自阳光的富含能量的生物聚合物可以转化为环境清洁和CO中性燃料。一组选择的光合微生物已经开发出提取和转移来自水的质子和电子到叶绿体氢化酶以产生分子H2燃料的能力。在这里，我们描述了C的发展和表征。rcinhardtii菌株，源自高H产量突变体Stm 6，其中引入了来自小球藻的HUP 1（己糖摄取蛋白）己糖同向转运体。分离的细胞系可以使用外部供应的葡萄糖在黑暗中进行异养生长。更重要的是，外部葡萄糖供应（1 mM）显示出增加菌株Stm 6 Glc 4中的H-2生产能力，接近于高H-2生产菌株Stm 6的150%。这为新的燃料生产工艺奠定了基础，其中H2O和葡萄糖可以同时用于H2生产。它也开辟了新的前景，未来的战略，提高生物H-2生产效率，在自然的白天/黑夜制度，并使用糖废料的能源生产的绿色藻类作为光合催化剂。(c)2007 Elsevier B. V.保留所有权利。
Phototrophic organisms use photosynthesis to convert solar energy into chemical energy. In nature, the chemical energy is stored in a diverse range of biopolymers. These sunlight-derived, energy-rich biopolymers can be converted into environmentally clean and CO, neutral fuels. A select group of photosynthetic microorganisms have developed the ability to extract and divert protons and electrons derived from water to chloroplast hydrogenase(s) to produce molecular H, fuel. Here, we describe the development and characterization of C. rcinhardtii strains, derived from the high H, production mutant Stm6, into which the HUP1 (hexose uptake protein) hexose symporter from Chlorella kessleri was introduced. The isolated cell lines can use externally supplied glucose for heterotrophic growth in the dark. More importantly, external glucose supply (1mM) was shown to increase the H-2 production capacity in strain Stm6Glc4 to similar to 150% of that of the high-H-2 producing strain, Stm6. This establishes the foundations for a new fuel production process in which H2O and glucose can simultaneously be used for H, production. It also opens new perspectives on future strategies for improving bio-H-2 production efficiency under natural day/night regimes and for using sugar waste material for energy production in green algae as photosynthetic catalysts. (c) 2007 Elsevier B.V. All rights reserved.