Phosphorylcholine-containing N-glycans of Trichinella spiralis:: identification of multiantennary lacdiNAc structures
Phosphorylcholine-containing N-glycans of Trichinella spiralis:: identification of multiantennary lacdiNAc structures
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DOI：
10.1093/glycob/10.9.941
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发表时间：
2000-09-01
期刊：
影响因子：
4.3
通讯作者：
Dell, A
中科院分区：
文献类型：
--
作者：
Morelle, W;Haslam, SM;Dell, A
虽然旋毛虫中存在磷酰胆碱(PC)已被证实，但含有PC的分子的确切结构尚不清楚。本文报道了用N-糖苷酶F从旋毛虫亲和纯化抗原中释放的N-糖链的结构研究，观察到三种N-糖链结构：高甘露糖型结构；完全修剪到甘露糖基核心的亚化学计量岩藻糖化结构；以及带有三甘露糖基核的结构，带有和不带有核心岩藻糖基化，携带1到8个N-乙酰己糖胺残基。在这三类多糖中，只有最后一类被可检测到的磷胆碱取代。
Although the presence of phosphorylcholine (PC) in Trichinella spiralis is well established, the precise structure of the PC-bearing molecules is not known. In this paper, we report structural studies of N-glycans released from T.spiralis affinity-purified antigens by peptide N-glycosidase F. Three classes of N-glycan structures were observed: high mannose type structures; those which had been fully trimmed to the trimannosyl core and were sub-stoichiometrically fucosylated; and those with a trimannosyl core, with and without core fucosylation, carrying between one and eight N-acetylhexosamine residues. Of the three classes of glycans, only the last was found to be substituted with detectable levels of phosphorylcholine.