PNGase F catalyzes de-N-glycosylation in a domestic microwave.
PNGase F catalyzes de-N-glycosylation in a domestic microwave.
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PNGase F 在家用微波炉中催化去 N-糖基化。
DOI：
10.1016/j.ab.2012.04.011
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发表时间：
2012
影响因子：
2.9
通讯作者：
Lee,RichardS
中科院分区：
文献类型：
--
作者：
Zhou,Hui;Briscoe,AndrewC;Froehlich,JohnW;Lee,RichardS
常见的去n -糖基化方案通常需要较长的潜伏期。虽然压力循环技术或科学的微波反应器可以加速这种酶的反应，但它们可能不容易获得。在这篇简短的报告中，我们采用了一种替代策略，使用标准的家用微波炉来进行去n -糖基化。模型糖蛋白（牛RNase B、牛胎蛋白和人IgG）和来自人血浆的复杂混合物在20min内完全去糖基化，对多糖或蛋白骨架没有明显的不良影响。这种新方法提供了一种简单而廉价的方法来实现快速的去n -糖基化。
Common de-N-glycosylation protocols usually require a lengthy incubation time. Although pressure cycling technology or scientific microwave reactors can accelerate this enzyme reaction, they may not be easily accessible. In this brief report, we employed an alternative strategy using a standard domestic microwave oven to perform the de-N-glycosylation. Model glycoproteins (bovine RNase B, bovine fetuin, and human IgG) and a complex mixture from human plasma were fully deglycosylated in 20min, without any apparent adverse affects on the glycans or protein backbones. This new method provides a simple and inexpensive solution to achieve rapid de-N-glycosylation.