Organotin compounds promote adipocyte differentiation as agonists of the peroxisome proliferator-activated receptor γ/retinoid x receptor pathway
Organotin compounds promote adipocyte differentiation as agonists of the peroxisome proliferator-activated receptor γ/retinoid x receptor pathway
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DOI：
10.1124/mol.104.008409
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发表时间：
2005-03-01
影响因子：
3.6
通讯作者：
Nishikawa, J
中科院分区：
文献类型：
--
作者：
Kanayama, T;Kobayashi, N;Nishikawa, J
核受体在维持内分泌系统、调节器官分化和胎儿发育中起重要作用。内分泌干扰物通过各种机制干扰内分泌系统，从而产生不利影响。为了评估内分泌干扰物对核受体的影响，我们开发了一种用于鉴定核受体激活剂的高通量方法。使用这个系统，我们发现三苯基锡和三丁基锡是过氧化物酶体增殖物激活受体（PPAR）γ和维甲酸X受体的激活剂。由于PPARgamma是脂肪细胞分化的主要调节剂，我们评估了有机锡化合物对前脂肪细胞3 T3-L1细胞的影响。我们发现，有机锡化合物刺激3 T3-L1细胞的分化以及脂肪细胞标记基因的表达。
Nuclear receptors play important roles in the maintenance of the endocrine system, regulation of organ differentiation, and fetal development. Endocrine disruptors exert their adverse effects by disrupting the endocrine system via various mechanisms. To assess the effects of endocrine disruptors on nuclear receptors, we developed a high-throughput method for identifying activators of nuclear receptors. Using this system, we found that triphenyltin and tributyltin were activators of peroxisome proliferator-activated receptor (PPAR) gamma and retinoid X receptor. Because PPARgamma is a master regulator of adipocyte differentiation, we assessed the effect of organotin compounds on preadipocyte 3T3-L1 cells. We found that organotin compounds stimulated differentiation of 3T3-L1 cells as well as expression of adipocyte marker genes.