Analysis of the crow lung transcriptome in response to infection with highly pathogenic H5N1 avian influenza virus.
Analysis of the crow lung transcriptome in response to infection with highly pathogenic H5N1 avian influenza virus.
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DOI：
10.1016/j.gene.2015.01.016
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发表时间：
2015-03
期刊：
影响因子：
3.5
通讯作者：
P. Vijayakumar;A. Mishra;P. Ranaware;A. P. Kolte;D. D. Kulkarni-D.;D. Burt;A. Raut
中科院分区：
文献类型：
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作者：
P. Vijayakumar;A. Mishra;P. Ranaware;A. P. Kolte;D. D. Kulkarni-D.;D. Burt;A. Raut
目前在亚洲流行的高致病性禽流感（HPAI）H5 N1病毒可在家禽和乌鸦等野生鸟类中引起严重疾病。然而，HPAIV感染乌鸦和其他野生鸟类的分子发病机制还不清楚。因此，作为探索它的一个步骤，使用高通量下一代测序（NGS）（GS FLX Titanium XLR 70）对感染HPAI H5 N1乌鸦分离株（A/Crow/India/11 TI 11/2011）的乌鸦肺进行全面的全局基因表达分析。乌鸦的参考基因组不可用，因此基于从头组装的转录组进行RNA测序分析。RNA测序结果显示，4052个基因在未感染的样本中唯一表达，6277个基因在HPAIV感染的样本中唯一表达，并且在两个样本中表达的6814个基因中，2279个基因显著差异表达。我们的转录组谱数据允许理解最近在乌鸦中致命的HPAIV爆发背后的分子机制的能力，直到最近，乌鸦被认为仅在鸡等鸡类鸟类中引起致命感染，而不是在野生鸟类中。差异表达基因的模式表明，这种H5 N1病毒分离株通过减弱干扰素（IFN）诱导的信号传导（可能是通过下调I型IFN的信号传导）来逃避宿主先天免疫应答（IFNAR 1和IFNAR 2）和II型IFN受体，细胞因子信号传导抑制因子1（SOCS 1）和SOCS 3的信号传导抑制因子的上调以及Toll样受体（TLR）表达的改变.这可能是乌鸦生病和死亡的原因。
The highly pathogenic avian influenza (HPAI) H5N1 virus, currently circulating in Asia, causes severe disease in domestic poultry as well as wild birds like crow. However, the molecular pathogenesis of HPAIV infection in crows and other wild birds is not well known. Thus, as a step to explore it, a comprehensive global gene expression analysis was performed on crow lungs, infected with HPAI H5N1 crow isolate (A/Crow/India/11TI11/2011) using high throughput next generation sequencing (NGS) (GS FLX Titanium XLR70). The reference genome of crow is not available, so RNA seq analysis was performed on the basis of a de novo assembled transcriptome. The RNA seq result shows, 4052 genes were expressed uniquely in noninfected, 6277 genes were expressed uniquely in HPAIV infected sample and of the 6814 genes expressed in both samples, 2279 genes were significantly differentially expressed. Our transcriptome profile data allows for the ability to understand the molecular mechanism behind the recent lethal HPAIV outbreak in crows which was, until recently, thought to cause lethal infections only in gallinaceous birds such as chickens, but not in wild birds. The pattern of differentially expressed genes suggest that this isolate of H5N1 virus evades the host innate immune response by attenuating interferon (IFN)-inducible signalling possibly by down regulating the signalling from type I IFN (IFNAR1 and IFNAR2) and type II IFN receptors, upregulation of the signalling inhibitors suppressor of cytokine signalling 1 (SOCS1) and SOCS3 and altering the expression of toll-like receptors (TLRs). This may be the reason for disease and mortality in crows.