DISTINCT PATTERNS OF CYCLIN D1 REGULATION IN MODELS OF LIVER-REGENERATION AND HUMAN LIVER
DISTINCT PATTERNS OF CYCLIN D1 REGULATION IN MODELS OF LIVER-REGENERATION AND HUMAN LIVER
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DOI：
10.1006/bbrc.1995.1548
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发表时间：
1995-04-17
影响因子：
3.1
通讯作者：
CERRA, FB
中科院分区：
文献类型：
--
作者：
ALBRECHT, JH;HU, MY;CERRA, FB
在肝再生模型和人类肝脏标本中检查了细胞周期蛋白 D1 和相关 cdks 的表达。在 70% 部分肝切除术后的小鼠肝脏中，细胞周期蛋白 D1 mRNA 和蛋白的诱导量增加了 20 倍以上，在达到峰值之前就开始了。 DNA合成。在正常大鼠肝脏中，细胞周期蛋白D1蛋白的基础水平显着高于小鼠。大鼠肝切除后，细胞周期蛋白 D1 mRNA 被诱导了 6 至 10 倍，而大鼠肝细胞中的蛋白质水平则变化了 20 倍。细胞周期蛋白 D1 的免疫沉淀显示其与再生肝脏中的 cdk4 相关，但与 cdk5 无关。在人肝活检标本中，在正常肝脏中可检测到细胞周期蛋白 D1 蛋白，并在移植后在有丝分裂活跃的肝脏中诱导 2 至 10 倍。这些结果表明，与大鼠肝切除模型相比，人肝脏中细胞周期蛋白 D1 蛋白的调节可能更接近于小鼠。此外，培养物中原代细胞中细胞周期蛋白的表达可能与体内观察到的显着不同。 (C) 1995 学术出版社
The expression of cyclin D1 and associated cdks was examined in models of liver regeneration and human liver specimens. In mouse liver after 70% partial hepatectomy, there was >20-fold induction of cyclin D1 mRNA and protein, beginning prior to peak. DNA synthesis. In normal rat liver, basal levels of cyclin D1 protein were significantly higher than in the mouse. After hepatectomy in the rat, cyclin D1 mRNA was induced 6- to 10-fold, while the protein levels changed 20-fold in rat hepatocytes. Immunoprecipitation of cyclin D1 demonstrated its association with cdk4 but not cdk5 in regenerating liver. In human liver biopsy specimens, cyclin D1 protein was detectable in normal liver and induced 2- to 10-fold in mitotically active liver following transplantation. These results suggest that the regulation of cyclin D1 protein in human liver may more closely parallel the mouse than the rat hepatectomy model. Furthermore, cyclin expression in primary cells in culture may differ significantly from that observed in vivo. (C) 1995 Academic Press, Inc.