COMPLETE NUCLEOTIDE-SEQUENCE OF CLOVER YELLOW MOSAIC-VIRUS RNA

COMPLETE NUCLEOTIDE-SEQUENCE OF CLOVER YELLOW MOSAIC-VIRUS RNA
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DOI:
10.1099/0022-1317-71-9-1913
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发表时间:
1990-09-01
影响因子:
3.8
通讯作者:
ABOUHAIDAR, MG
ABOUHAIDAR, MG
中科院分区:
医学3区
文献类型:
--
作者:
SIT, TL;WHITE, KA;ABOUHAIDAR, MG

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从三叶草黄花叶病毒的cDNA克隆和流出的cDNA转录本中测定了其全基因组RNA的序列。基因组RNA长度为7015个核苷酸[不包括3“poly(A)尾],具有6个长度大于150个核苷酸的开放阅读框(ORF)。前5个ORF分别编码Mr为191 K、26 K、12 K、6.5K和28 K的蛋白质。第六个ORF完全位于ORF 1内,编码Mr 14 K蛋白。衣壳蛋白编码区(Mr 23 K)位于编码Mr 28 K蛋白的ORF 5内。ORF 1 ~ 3和ORF 5编码的蛋白质与其他马铃薯X病毒属的蛋白质有很强的同源性,特别是与番木瓜花叶病毒属的蛋白质同源性更高。
The entire genomic RNA of clover yellow mosaic virus was sequenced from cDNA clones and run-off cDNA transcripts. The genomic RNA is 7015 nucleotides in length [excluding a 3'' poly(A) tail], with six open reading frames (ORFs) greater than 150 nucleotide in length. The first five ORFs encode proteins of Mr 191K, 26K, 12K, 6.5K and 28K, respectively. The sixth ORF lies completely within ORF1 and codes for a protein of Mr 14K. The capsid protein coding region (Mr 23K) is found within ORF5 which encodes the Mr 28K protein. Products encoded by ORFs 1 to 3 and ORF5 show strong homology with proteins of other potexviruses, especially papaya mosaic virus.