Preservation of rat cremaster muscle microcirculation after prolonged cold storage and transplantation
Preservation of rat cremaster muscle microcirculation after prolonged cold storage and transplantation
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DOI：
10.1016/j.jss.2005.05.027
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发表时间：
2006-03-01
影响因子：
2.2
通讯作者：
Egbrink, MGAO
中科院分区：
文献类型：
--
作者：
Bastiaanse, J;Nanhekhan, LV;Egbrink, MGAO
背景资料。微血管手术修复复杂的缺血区涉及一段缺血期，这可能会导致缺血/再灌流损伤导致的皮瓣失效。我们评估了大鼠提睾肌移植在HTK-Bretschneider溶液(HTK)中长时间冷藏后的微血管后果。材料和方法。在HTK或生理盐水中冷藏(4℃)8或24小时后，立即或在提睾肌移植手术。活体显微镜用于量化移植后的毛细血管血流和静脉白细胞与内皮细胞的相互作用。移植过程本身导致了50-65分钟的缺血。直接移植后，毛细血管灌注率为对照组的90%。在HTK或生理盐水中冷藏8h后的移植并没有恶化毛细血管的灌流。保存24 h后，HTK组保存毛细血管灌注率明显优于生理盐水组(HTK组为对照组的76~83%，生理盐水组为30%)。即刻移植引起的白细胞黏附略有增加。在任何一种液体中长期冷藏都会导致血流速度减慢(定性观察)和水肿的形成，这阻碍了对白细胞-内皮相互作用的量化。即使在HTK冷藏8h或24h后，大鼠提睾肌移植仍成功，毛细血管灌流良好。HTK较生理盐水保存毛细血管灌流效果更好。(C)2006 Elsevier Inc.保留所有权利。
Background. Microvascular surgery for the reconstruction of complex defects involves an ischemic period, which may cause flap failure as the result of ischemia/reperfusion injury. We assessed the microvascular consequences of rat cremaster muscle transplantation after prolonged periods of cold storage in HTK-Bretschneider solution (HTK).Materials and methods. Cremaster muscle transplantations were performed immediately or after 8 or 24 h of cold storage (4 degrees C) in HTK or saline. Intravital microscopy was used to quantify capillary perfusion and venular leukocyte-endothelium interactions following transplantation.Results. The transplantation procedure itself resulted in 50-65 min of ischemia. After direct transplantation, capillary perfusion was 90% of control. Transplantation after 8 h of cold storage in either HTK or saline did not deteriorate capillary perfusion. When the tissue was stored for 24 h, HTK was superior to saline in preserving capillary perfusion (HTK: 76-83% of control, saline: 30%). Immediate transplantation induced a small increase in leukocyte adhesion. Prolonged cold storage in either fluid resulted in reduced flow velocities (qualitative observations) and edema formation, which hampered quantification of leukocyte-endothelium interactions.Conclusions. Even after 8 or 24 h of cold storage in HTK, transplantation of rat cremaster muscle was successful with good capillary perfusion. Capillary perfusion was better preserved in HTK than in saline. (c) 2006 Elsevier Inc. All rights reserved.