Interlaboratory studies on in vitro test methods for estimating in vivo resorption of calcium phosphate ceramics
Interlaboratory studies on in vitro test methods for estimating in vivo resorption of calcium phosphate ceramics
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DOI：
10.1016/j.actbio.2015.07.040
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发表时间：
2015-10-01
影响因子：
9.7
通讯作者：
Bohner, Marc
中科院分区：
文献类型：
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作者：
Ito, Atsuo;Sogo, Yu;Bohner, Marc
提出了一种测量相对溶解速率以评价磷酸钙基陶瓷可吸收性的标准方法。在9个研究中心，将磷酸三钙（TCP）、镁取代的TCP（MgTCP）和锌取代的TCP（ZnTCP）溶解在pH 4.0、5.5或7.3的不含钙和磷酸根离子的缓冲溶液中。初始溶出速率的相对值（相对溶出速率）在各中心之间具有良好的一致性。ZnTCP的相对溶出速率与体外吸收陷窝的相对体积相一致。在比较具有不同Mg含量和相似孔隙率的微孔MgTCP的情况下，相对溶解速率与体内相对再吸收体积一致。然而，在微孔TCP和MgTCP之间比较的情况下，由于Mg介导的TCP溶解度降低叠加在Mg介导的再吸收量增加上，相对溶解速率与体内相对再吸收体积的一致性较差。在比较具有不同孔隙率的TCP的情况下，无法得出相对溶解速率是否可以预测体内相对再吸收体积的明确结论。相对溶解速率可用于预测磷酸钙的相对再吸收量，在所比较的材料中的差异对再吸收过程（例如再吸收细胞的数量和活性）几乎没有影响的条件下，具有不同溶解度的磷酸钙基陶瓷。磷酸盐尽管磷酸钙的可吸收性通常在体内进行评价，但建立一种可预测体内吸收的标准体外方法有利于加速新型可吸收磷酸钙材料的开发和商业化，并减少动物使用。然而，只有少数研究提出了这样一种体外方法，在体外和体内吸收之间进行了直接比较。我们在这里提出了一种基于测量溶出速率的体外方法。该方法的有效性和局限性进行了评估，通过国际循环试验，以及与体内吸收研究的比较，为未来的标准化。这项研究是作为凡尔赛先进材料和标准项目（VAMAS）之一进行的。(C)2015 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
A potential standard method for measuring the relative dissolution rate to estimate the resorbability of calcium-phosphate-based ceramics is proposed. Tricalcium phosphate (TCP), magnesium-substituted TCP (MgTCP) and zinc-substituted TCP (ZnTCP) were dissolved in a buffer solution free of calcium and phosphate ions at pH 4.0, 5.5 or 7.3 at nine research centers. Relative values of the initial dissolution rate (relative dissolution rates) were in good agreement among the centers. The relative dissolution rate coincided with the relative volume of resorption pits of ZnTCP in vitro. The relative dissolution rate coincided with the relative resorbed volume in vivo in the case of comparison between microporous MgTCPs with different Mg contents and similar porosity. However, the relative dissolution rate was in poor agreement with the relative resorbed volume in vivo in the case of comparison between microporous TCP and MgTCP due to the superimposition of the Mg-mediated decrease in TCP solubility on the Mg-mediated increase in the amount of resorption. An unambiguous conclusion could not be made as to whether the relative dissolution rate is predictive of the relative resorbed volume in vivo in the case of comparison between TCPs with different porosity. The relative dissolution rate may be useful for predicting the relative amount of resorption for calcium-phosphate-based ceramics having different solubility under the condition that the differences in the materials compared have little impact on the resorption process such as the number and activity of resorbing cells.Statement of significanceThe evaluation and subsequent optimization of the resorbability of calcium phosphate are crucial in the use of resorbable calcium phosphates. Although the resorbability of calcium phosphates has usually been evaluated in vivo, establishment of a standard in vitro method that can predict in vivo resorption is beneficial for accelerating development and commercialization of new resorbable calcium phosphate materials as well as reducing use of animals. However, there are only a few studies to propose such an in vitro method within which direct comparison was carried out between in vitro and in vivo resorption. We propose here an in vitro method based on measuring dissolution rate. The efficacy and limitations of the method were evaluated by international round-robin tests as well as comparison with in vivo resorption studies for future standardization. This study was carried out as one of Versailles Projects on Advanced Materials and Standards (VAMAS). (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.