Metabolism of mRNA from the transforming region of adenovirus 2.
Metabolism of mRNA from the transforming region of adenovirus 2.
复制标题
腺病毒2转化区mRNA的代谢。
DOI：
10.1101/sqb.1980.044.01.048
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
DarnellJr,JE
中科院分区：
文献类型：
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作者：
Wilson,MC;Nevins,JR;Blanchard,JM;Ginsberg,HS;DarnellJr,JE
图1给出了在转化细胞以及早期和晚期腺病毒感染中起作用的转录单位的基因组位置。我们已经证明，在Ad 2基因组的左侧11%内，在感染早期有活性的启动子位于大约1.5个图单位以及5个图单位处，并且在感染后期它们位于大约9个图单位处（Wilson等，1979）。通过电子显微镜和RNA-DNA杂交体的凝胶分析确定mRNA中存在的序列和剪接排列（Kitchingman et al. 1977; Berk and Sharp 1978）。早先，我们通过将标记不同时间的mRNA与代表转录单位a的DNA片段杂交，证明了不同大小的mRNA及其相对比例。
RESULTSThe genomic location of the transcriptional units that function in transformed cells as well as in early and late adenoviral infection is given in Figure 1. We have demonstrated that, within the left 11% of the Ad2 genome, promoters that are active early in infection are located at approximately 1.5 map units, as well as 5 map units, and late in infection they are located at approximately 9 map units (Wilson et al. 1979). The sequences and splicing arrangements that are present in mRNA were determined by electron microscopy and by gel analysis of RNA-DNA hybrids (Kitchingman et al. 1977; Berk and Sharp 1978). Earlier we had demonstrated different-size mRNAs and their relative proportions by hybridizing mRNA labeled for various times to the DNA fragments representing transcription units a.