STEREOLOGICAL MEASUREMENT OF CELLULAR AND SUBCELLULAR HYPERTROPHY AND HYPERPLASIA IN THE PAPILLARY-MUSCLE OF ADULT-RAT
STEREOLOGICAL MEASUREMENT OF CELLULAR AND SUBCELLULAR HYPERTROPHY AND HYPERPLASIA IN THE PAPILLARY-MUSCLE OF ADULT-RAT
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DOI：
10.1016/0022-2828(80)90080-2
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发表时间：
1980-01-01
影响因子：
5
通讯作者：
LOUD, AV
中科院分区：
文献类型：
--
作者：
ANVERSA, P;OLIVETTI, G;LOUD, AV
通过组织、细胞和亚细胞形态计量学的结合，观察了乳头肌在51%诱导生长后的结构变化。形态计量学核计数比较不同细胞群体的适应性反应，内皮细胞增殖48%，结缔组织细胞35%，心肌细胞核数量无变化。衡量细胞肥大的单位核细胞体积增加，内皮细胞为35%，结缔组织细胞为%，心肌细胞为53%。乳头肌长度、平均肌细胞数和毛细血管网无明显变化。总毛细血管体积、管腔内皮细胞表面和管腔内皮细胞表面分别显著增加59%、38%和43%。乳头肌中各细胞群的体积、表面积和胞浆细胞器数量均有不同程度的增加，并伴有细胞增殖和/或肥大。内皮细胞中的微吞饮小泡增加了73%；成纤维细胞中的粗面内质网增加。150%；S[平滑]ER和T系统在心肌细胞中，每个.apprx。内皮细胞、成纤维细胞和肌细胞线粒体数量分别为54、78和65%。由于正常大小成分的侧向复制，肌细胞内的肌原纤维网络体积增加了67%，表面积增加了55%，肌原纤维分支长度增加了82%，肌节单位总数增加了86%。为正常乳头肌和肥大乳头肌的功能研究提供了形态学基础。
Structural changes in the papillary muscle were examined following an induced growth of 51% by integrating tissue, cellular and subcellular morphometry. Comparison of the adaptive response in different cell populations by morphometric nuclear enumeration demonstrated 48% hyperplasia of endothelial cells, 35% for connective tissue cells and no change in the number of myocyte nuclei. Increases in the cell volume per nucleus, a measure of cellular hypertrophy, were 35% for endothelial cells, 64% for connective tissue cells and 53% for myocytes. No significant changes were found in the lengths of the papillary muscle, the average myocyte or the capillary network. Total capillary volume and the luminal and abluminal endothelial surfaces were significantly increased by 59, 38 and 43%, respectively. Each cell population in the papillary muscle showed varying increases in the volumes, surface areas and number of cytoplasmic organelles accompanying cellular hyperplasia and/or hypertrophy. Micropinocytotic vesicles in endothelial cells increased 73%; RER [rough endoplasmic reticulum] in fibroblasts .apprx. 150%; S [smooth] ER and T-system in myocytes, each .apprx. 100% and the numbers of mitochondria in endothelium, fibroblasts and myocytes, 54, 78 and 65%. As a result of a lateral duplication of normal sized components, the myofibrillar network in myocytes increased 67% in volume, 55% in surface area, 82% in the length of myofibril branches and 86% in the overall number of its sarcomere units. A morphological basis for functional studies of normal and hypertrophied papillary muscle is provided.