Comparative efficacy of replicating smallpox vaccine strains in a Murine Challenge Model
Comparative efficacy of replicating smallpox vaccine strains in a Murine Challenge Model
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DOI：
10.1016/j.vaccine.2005.02.005
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发表时间：
2005-05-20
期刊：
影响因子：
5.5
通讯作者：
Ulaeto, DO
中科院分区：
文献类型：
--
作者：
Phelps, A;Gates, AJ;Ulaeto, DO
目前，人们对人类易受天花病毒（VARV）生物战或生物恐怖袭击的脆弱性表示极大关注。传统的天花疫苗是使用感染痘苗病毒（VACV）的反刍动物的淋巴液制造的。然而，这些生产方法不符合当前的疫苗标准，特别是因为驯养反刍动物中出现了传染性海绵状脑病。本研究在小鼠致死攻击模型中检验了不同来源的 Lister (Elstree) 疫苗株的保护功效。从美国典型培养物保藏中心 (ATCC) 获得的利斯特材料与从疫苗库存获得的相同菌株之间观察到功效存在相当大的差异。根据传统疫苗历史库存中的多个批次的基准，对一种新的、组织培养衍生的李斯特疫苗进行评估。历史疫苗和新疫苗之间观察到明显的质量差异。不同批次的传统疫苗之间存在统计学上的显着差异，而组织培养生产的疫苗的功效也在这个范围内。 Crown 版权所有 (c) 2005 由 Elsevier Ltd 出版。保留所有权利。
There is currently considerable concern about the vulnerability of human populations to biowarfare or bioterrorist attacks with variola virus (VARV). Traditional smallpox vaccines were manufactured using the lymph of ruminants infected with the vaccinia virus (VACV). However, these production methods do not meet current standards for vaccines, especially since the emergence of transmissable spongiform encephalopathies in domesticated ruminants. This study has examined the protective efficacy of the Lister (Elstree) vaccine strain from various sources in a murine lethal challenge model. Considerable variation in efficacy is observed between the Lister material obtained from the American Type Culture Collection (ATCC) and the same strain obtained from vaccine stockpiles. A new, tissue-culture derived Lister vaccine is assessed against a bench-mark of multiple lots from a historical stockpile of the traditional vaccine. Apparent qualitative differences are observed between historical and new vaccines. Statistically significant differences are observed between different batches of the traditional vaccine, and the efficacy of the tissue-culture produced vaccine falls within this range. Crown Copyright (c) 2005 Published by Elsevier Ltd. All rights reserved.