In vivo subcellular resolution optical imaging in the lung reveals early metastatic proliferation and motility.
In vivo subcellular resolution optical imaging in the lung reveals early metastatic proliferation and motility.
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DOI：
10.1080/21659087.2015.1086613
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发表时间：
2015-09
期刊：
影响因子：
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通讯作者：
Condeelis J
中科院分区：
文献类型：
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作者：
Entenberg D;Rodriguez-Tirado C;Kato Y;Kitamura T;Pollard JW;Condeelis J
为了更好地了解乳腺癌转移细胞种植，我们使用了多光子显微镜和真空稳定窗口，从而不需要复杂的注册软件、视频速率显微镜或专门的门控电子设备来观察活的、呼吸的肺内肿瘤细胞种植的初始步骤。我们观察到，在到达肺部时，肿瘤细胞仅在毛细血管中发现，完全填满它们的体积，并显示出最初的高水平突起活动，随着时间的推移显著减少。此外，我们观察到，在同一时期，位置稳定性也随之增加。我们使用了几种大多数成像实验室都可以使用的技术来优化信噪比和分辨率，这使我们能够以亚细胞分辨率报告第一次直接观察到肺内转移的肿瘤细胞的到达、增殖和运动。
To better understand breast cancer metastatic cell seeding, we have employed multiphoton microscopy and a vacuum stabilized window which eliminates the need for complex registration software, video rate microscopy or specialized gating electronics to observe the initial steps of tumor cell seeding within the living, breathing lung. We observe that upon arrival to the lung, tumor cells are found exclusively in capillary vessels, completely fill their volume and display an initial high level of protrusive activity that dramatically reduces over time. Further, we observe a concomitant increase in positional stability during this same period. We employ several techniques accessible to most imaging labs for optimizing signal to noise and resolution which enable us to report the first direct observation, with subcellular resolution, of the arrival, proliferation, and motility of metastatic tumor cells within the lung.