Peripheral blood neutrophils in inflammatory bowel disease: morphological evidence of in vivo activation in active disease

Peripheral blood neutrophils in inflammatory bowel disease: morphological evidence of in vivo activation in active disease
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炎症性肠病中的外周血中性粒细胞:活动性疾病中体内激活的形态学证据

DOI:
--
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发表时间:
1991
影响因子:
4.6
通讯作者:
Y.
Y.
中科院分区:
医学3区
文献类型:
--
作者:
D. McCarthy;D. Rampton;Y.

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用体外固定的外周静脉血制备甲苯胺蓝白细胞制剂,通过定量光学显微镜检查,寻找炎症性肠病(IBD)患者循环中性粒细胞在体内活化的形态证据。活动期克罗恩病患者外周血中球形(未激活)中性粒细胞比例(73%;46-96(中位数;范围),n=11)低于稳定期克罗恩病(90%;45-99;n=18,P<0.01)和正常人(94%;44-98;n=13,P<0.05)。活动期溃疡性结肠炎患者中性粒细胞数(77%;13-96;n=17)明显少于静止期结肠炎患者(88%;57-99,n=13,P<0.1)和正常人(P<0.05)。激活的中性粒细胞出现在活动期IBD患者的循环池中,并可通过外周静脉血白细胞制剂的光学显微镜检测到。
Morphological evidence of activation in vivo of circulating neutrophils in patients with inflammatory bowel disease (IBD) was sought by quantitative light microscope examination of toluidine bluestained leucocyte preparations made from peripheral venous bloods that had been fixed immediately ex vivo. The proportion of spherical (unactivated) circulating neutrophils was reduced in active Crohn's disease (73%; 46–96 (median; range), n= 11) compared with inactive Crohn's (90%; 45–99; n= 18, P<0.01) and normal subjects (94%; 44–98; n=13, P<0.05). There tended to be fewer spherical neutrophils in active ulcerative colitis (77%; 13–96; n= 17) than in quiescent colitis (88%; 57–99, n= 13, P<0.1) or normal subjects (P<0.05). Activated neutrophils occur in the circulating pool of patients with active IBD and can be detected by light microscopy of peripheral venous blood leucocyte preparations.
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