Arginine as an Enhancer in Rose Bengal Photosensitized Corneal Crosslinking
Arginine as an Enhancer in Rose Bengal Photosensitized Corneal Crosslinking
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精氨酸作为孟加拉红光敏角膜交联的增强剂
DOI：
10.1167/tvst.9.8.24
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发表时间：
2020
影响因子：
3
通讯作者：
I. Kochevar
中科院分区：
文献类型：
--
作者：
C. Wertheimer;Bryan Mendes;Qing Pei;Katharina Brandt;I. Kochevar
目的利用孟加拉玫瑰(RB)和绿光进行氧非依赖性角膜交联术(CXL)可能具有独特的临床应用。这些研究旨在深入了解精氨酸(Arg)增强的厌氧交联过程，最大限度地提高交联效率，并测试临床上可行的无氧CXL方法。方法用1mmRb和532 nm激光对兔角膜进行体外处理。在吸收光谱监测下，Rb光解被用来优化Arg的浓度，并建立了辐照和再染色的方案。通过线性拉伸强度测量确定了最小有效绿光通量。荧光显微镜下观察Rb对基质的渗透。为了有利于无氧途径，在照射过程中使用了隐形眼镜来最小化基质中的氧气水平。采用末端脱氧核苷酸转移酶介导的三磷酸脱氧尿苷缺口末端标记法(TUNEL)评价基质细胞的毒性。结果Rb在200 mM Arg时达到最大光解效率的75%，最佳光解通量增量为32.7J/cm2。角膜硬化的最小有效剂量为65.4J/cm2。隐形眼镜的放置促进了非氧依赖的角膜硬化，类似于在孤立的缺氧角膜上所获得的结果。在有Arg存在的情况下，Rb对基质的渗透被限制在∼120um，比没有Arg时深约25%。基质细胞的毒性仅限于Rb和Arg的穿透深度。结论RB-CXL在角膜中的氧非依赖途径得到了表征和优化，包括一种可能的临床应用方案。翻译相关性氧非依赖性RB-CXL是一种高效、有效的治疗方法，可进一步开发用于独特的临床应用。
Purpose Oxygen-independent cornea crosslinking (CXL) using rose bengal (RB) and green light may have unique clinical applications. These studies were designed to gain insight into the arginine (arg)-enhanced anaerobic crosslinking process, to maximize crosslinking efficiency, and to test a clinically feasible method for oxygen-free CXL. Methods Rabbit corneas were treated ex vivo using 1 mM RB and 532 nm light. RB photodecomposition, monitored by absorption spectrophotometry, was used to optimize arg concentration and to develop an irradiation and re-dying protocol. The minimal effective green light fluence was identified by linear tensile strength measurements. RB penetration into the stroma was determined by fluorescence microscopy. To favor the anaerobic pathway, a contact lens was used to minimize stromal oxygen level during irradiation. Stromal cell toxicity was evaluated by a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay. Results RB photodecomposition reached 75% of its maximal effect at 200 mM arg and the optimal fluence increment was 32.7 J/cm2. The minimal effective fluence for cornea stiffening was 65.4 J/cm2. Placement of a contact lens promoted oxygen-independent cornea stiffening, similar to that obtained on isolated, oxygen-deprived cornea. RB penetration into the stroma with arg present was limited to ∼120 µm, about 25% deeper than without arg. Stromal cell toxicity was limited to the depth of RB and arg penetration. Conclusions An oxygen-independent pathway in cornea for RB-CXL was characterized and optimized, including a possible clinical protocol for its use. Translational Relevance Oxygen-independent RB-CXL is an efficient and effective process that can be developed further for unique clinical applications.
局部应用 L-精氨酸可阻断 hSVCT2 转基因小鼠晶状体中抗坏血酸引起的晚期糖化。
影响因子：
2.2
作者：
Fan,Xingjun;Xiaoqin,Liu;Potts,Breshey;Strauch,ChristopherM;Nemet,Ina;Monnier,VincentM
通讯作者：
Monnier,VincentM
影响因子：
4.4
作者：
Cherfan, Daniel;Verter, E. Eri;Kochevar, Irene E.
通讯作者：
Kochevar, Irene E.