Catalytic effect of nanogold on Cu(II)-N2H4 reaction and its application to resonance scattering immunoassay.
Catalytic effect of nanogold on Cu(II)-N2H4 reaction and its application to resonance scattering immunoassay.
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纳米金对Cu(II)-N2H4反应的催化作用及其在共振散射免疫分析中的应用
DOI：
10.1021/ac801647b
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发表时间：
2008-10
影响因子：
7.4
通讯作者：
中科院分区：
文献类型：
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在EDTA-NaOH介质中，纳米金强烈催化肼（N2 H4）与Cu（II）的缓慢反应生成Cu粒子，在602 nm处产生强的共振散射（RS）峰。在602 nm处增加的RS强度（Δ I（RS））在0.008-2.64 nM范围内与纳米金浓度呈线性关系，检测限为1.0 pM Au。由初始速率法得到的反应速率方程为V（Cu）= K（Cu）[C（Cu（II））]（2）C（OH）（1）C（Au）（1）C（N2）H4（1），表观活化能为38 kJ × mol（-1），并对催化反应机理进行了探讨。建立了免疫纳米金-催化共振散射光谱（RSS）检测微量白蛋白（Malb）的方法。在pH5.0的柠檬酸-Na_2HPO_4缓冲溶液中，AuMalb发生非特异性聚集。加入Malb后，它与探针反应，在溶液中形成分散的AuMalb-Malb免疫复合物。离心后，得到含AuMalb-Malb的上清液，该上清液对N2 H4-Cu（II）反应具有催化作用，产生大的Cu颗粒，导致I（602 nm）增加。在0.4 ~ 460 pg × mL（-1）范围内，Malb浓度与602 nm处的RS强度呈线性关系，回归方程为Δ I（602 nm）= 0.3713 C（Malb）+ 7.2，相关系数为0.9981，检出限为0.1 pg × mL（-1）Malb。方法用于健康人尿样中Malb的检测，结果满意。
In the medium of EDTA-NaOH, nanogold strongly catalyzed the slow reaction between hydrazine (N2H4) and Cu(II) to form Cu particles, which exhibited a strong resonance scattering (RS) peak at 602 nm. The increased RS intensity at 602 nm (DeltaI(RS)) was linear to the nanogold concentration in the range of 0.008-2.64 nM, with a detection limit of 1.0 pM Au. The rate equation obtained by the initial rate procedure was V(Cu) = K(Cu)[C(Cu(II))](2)C(OH)(1)C(Au)(1)C(N2)H4(1), with an apparent activation energy of 38 kJ x mol(-1), and the catalytic reaction mechanism was also discussed. An immunonanogold-catalytic resonance scattering spectral (RSS) assay was established for detection of microalbumin (Malb), using 10 nm nanogold to label goat antihuman Malb to obtain an immunonanogold probe (AuMalb) for Malb. In pH 5.0 citric acid-Na2HPO4 buffer solution, the AuMalb aggregated nonspecifically. Upon addition of Malb, it reacted with the probe to form dispersive AuMalb-Malb immunocomplex in the solution. After centrifugation, the supernatant containing AuMalb-Malb was obtained, and exhibited a catalytic effect on the reaction of N2H4-Cu(II) to produce large Cu particles that resulted in the I(602 nm) increasing. The increased RS intensity at 602 nm (DeltaI(602 nm)) was linear to Malb concentration (C(Malb)) in the range of 0.4 to 460 pg x mL(-1), with the regression equation of DeltaI(602 nm) = 0.3713 C(Malb) + 7.2, correlation coefficient of 0.9981 and detection limit of 0.1 pg x mL(-1) Malb. The proposed method was applied to detect Malb in healthy human urine samples, with satisfactory results.