RAPID STIMULATION OF FLUID-PHASE ENDOCYTOSIS AND EXOCYTOSIS BY INSULIN, INSULIN-LIKE GROWTH FACTOR-I, AND EPIDERMAL GROWTH-FACTOR IN KB CELLS
RAPID STIMULATION OF FLUID-PHASE ENDOCYTOSIS AND EXOCYTOSIS BY INSULIN, INSULIN-LIKE GROWTH FACTOR-I, AND EPIDERMAL GROWTH-FACTOR IN KB CELLS
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DOI：
10.1016/0014-4827(88)90379-5
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发表时间：
1988-09-01
影响因子：
3.7
通讯作者：
SAKAI, H
中科院分区：
文献类型：
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作者：
MIYATA, Y;HOSHI, M;SAKAI, H
以辣根过氧化物酶（HRP）为标记物，观察了生长因子对人表皮样癌KB细胞液相内吞和胞吐的影响。胰岛素、胰岛素样生长因子-I（IGF-I）和表皮生长因子（EGF）促进HRP积累。它们还刺激预载的HRP从细胞中流出。从这些结果可以看出，这些生长因子刺激HRP的流入和流出，因为积累速率是流入速率和流出速率的总和。在加入生长因子的2-4分钟内迅速诱导HRP积累和HRP流出的刺激，并持续至少60分钟。10-7，1倍10-9和5倍。10-10 M。α IR-3（抗I型IGF受体抗体）完全阻断IGF-I对HRP积累的刺激，但非常轻微地抑制胰岛素的刺激。528 IgG（抗EGF受体抗体）抑制EGF对HRP积累的刺激。这些结果表明，这些生长因子中的每一种刺激由相应的（同源）生长因子受体介导的HRP积累。液相流入和流出的快速刺激可能构成对生长因子的常见早期细胞反应之一。
Effects of growth factors on fluid-phase endocytosis and exocytosis in human epidermoid carcinoma KB cells were examined by measuring horseradish peroxidase (HRP) as a marker. Insulin, insulin-like growth factor-I (IGF-I), and epidermal growth factor (EGF) promoted HRP accumulation. They also stimulated the efflux of the preloaded HRP from the cells. From these results it follows that these growth factors stimulate the influx as well as the efflux of HRP, because the accumulation rate is the sum of the influx rate and the efflux rate. The stimulation of both HRP accumulation and HRP efflux was rapidly induced within 2-4 min of the addition of growth factors and persisted for at least 60 min. The concentrations eliciting half-maximal stimulatory effects of insulin, IGF-I, and EGF were about 5 .times. 10-7, 1 .times. 10-9, and 5 .times. 10-10 M, respectively. .alpha.IR-3 (anti-type I IGF receptor antibody) completely blocked the stimulation of HRP accumulation by IGF-I but very slightly inhibited the stimulation by insulin. The 528 IgG (anti-EGF receptor antibody) inhibited the stimulation of HRP accumulation by EGF. These results indicated that each of these growth factors stimulates the HRP accumulation mediated by the corresponding (homologous) growth factor receptors. The rapid stimulation of fluid-phase influx and efflux may constitute one of the common early cellular responses to growth factors.