In vivo transcriptomic analysis of Beauveria bassiana reveals differences in infection strategies in Galleria mellonella and Plutella xylostella
In vivo transcriptomic analysis of Beauveria bassiana reveals differences in infection strategies in Galleria mellonella and Plutella xylostella
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白僵菌体内转录组分析揭示大蜡螟和小菜蛾感染策略的差异
DOI：
10.1002/ps.5266
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发表时间：
2019
影响因子：
4.1
通讯作者：
Wang Yulong
中科院分区：
文献类型：
--
作者：
Zhou Qiumei;Shao Ying;Chen Anhui;Li Wanzhen;Wang Jiuxiang;Wang Yulong
背景昆虫进化出了各种防御机制来对抗真菌感染，真菌也发展了多种策略来克服昆虫的免疫防御反应。结果球孢白僵菌在感染梅氏白僵菌和小菜蛾后12、24和48 h进行转录谱分析，分别检测到540、847和932个差异表达基因。功能分类表明，这些基因大多参与核糖体、氮代谢和氧化磷酸化途径。发现了31个差异表达的毒力基因(包括与黏附、降解、寄主定植和杀死以及次生代谢相关的基因)，这表明该真菌在不同害虫侵染过程中使用了不同的分子机制，并通过干扰creA和fkh2进一步证实了这一点。毒力测定结果表明，ΔA和Δfkh2菌株的致病力较强。球孢霉在侵染过程中，其半数致死时间(LT50)值(与对照菌株相比)有明显的倍增变化。Mellonella(ΔCREA：1.38倍；ΔFKH2：1.18倍)和P.小菜蛾(Δcra：1.44倍；Δfkh2：2.25倍).creA在侵染过程中表达水平较高。梅隆与P。而Fkh2则表现出相反的表达模式，说明creA和Fkh2在B中的作用不同。他对G的感染很敏感。梅隆和P。结论本研究结果表明，B.球孢菌调节不同的基因感染不同的昆虫，促进了对白僵菌与害虫相互作用的分子机制的了解。2018年中国化学工业学会
BACKGROUNDInsect pests have evolved various defense mechanisms to combat fungal infection, and fungi have developed multiple strategies to overcome the immune defense responses of insects. However, transcriptomic analysis of fungal strategies for infecting different pests has not been reported.RESULTSTranscriptomic profiling ofBeauveria bassianawas performed at 12, 24 and 48 h after infectingGalleria mellonellaandPlutella xylostella, and 540, 847 and 932 differentially expressed genes were detected, respectively. Functional categorization showed that most of these genes are involved in the ribosome, nitrogen metabolism and oxidative phosphorylation pathways. Thirty‐one differentially expressed virulence genes (including genes involved in adhesion, degradation, host colonization and killing, and secondary metabolism) were found, suggesting that different molecular mechanisms were used by the fungus during the infection of different pests, which was further confirmed by disruptingcreAandfkh2. Virulence assay results showed thatΔcreAandΔfkh2strains ofB. bassianahad distinct fold changes in their 50% lethal time (LT50) values (compared with the control stains) during infection ofG. mellonella(ΔcreA: 1.38‐fold >Δfkh2: 1.18‐fold) andP. xylostella(ΔcreA: 1.44‐fold <Δfkh2: 2.25‐fold).creAwas expressed at higher levels during the infection ofG. mellonellacompared withP. xylostella, whereasfkh2showed the opposite expression pattern, demonstrating thatcreAandFkh2have different roles inB. bassianaduring the infection ofG. mellonellaandP. xylostella.CONCLUSIONThese findings demonstrate thatB. bassianaregulates different genes to infect different insects, advancing knowledge of the molecular mechanisms ofBeauveria–pest interactions. © 2018 Society of Chemical Industry