Antibacterial Mode of Action of Cinnamomum verum Bark Essential Oil, Alone and in Combination with Piperacillin, Against a Multi-Drug-Resistant Escherichia coli Strain
Antibacterial Mode of Action of Cinnamomum verum Bark Essential Oil, Alone and in Combination with Piperacillin, Against a Multi-Drug-Resistant Escherichia coli Strain
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DOI：
10.4014/jmb.1407.07054
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发表时间：
2015-08-01
影响因子：
2.8
通讯作者：
Lim, Swee Hua Erin
中科院分区：
文献类型：
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作者：
Yap, Polly Soo Xi;Krishnan, Thiba;Lim, Swee Hua Erin
本研究的目的是研究肉桂皮精油（CB）的作用机制，单独使用时，也与哌拉西林组合，其抗β-内酰胺酶TEM-1质粒赋予大肠杆菌J53 R1的抗菌和协同活性。精油和抗生素的这种组合的细菌的活菌计数在20小时时显示出完全的杀灭特征，并通过减少细菌细胞数进一步证实了其协同作用。当针对十二烷基硫酸钠进行测试时，CB对经处理的培养物的细胞膜渗透性的稳定性分析显示，细菌细胞膜被精油破坏。扫描电镜观察和细菌表面电荷测量也表明CB引起不可逆的膜损伤，降低细菌表面电荷。此外，还对大肠杆菌[pSB 1075]和大肠杆菌E. coli [pSB 401]中的酶活性降低，表明可能存在群体感应（quorum sensing，QS）抑制剂。气相色谱-质谱联用分析表明，肉桂挥发油中主要成分为反式肉桂醛（72.81%）、苯甲醇（12.5%）和丁香酚（6.57%）。CB具有逆转E.大肠杆菌J53 R1通过两种途径对哌拉西林产生抗性：外膜通透性的改变或细菌QS抑制。
This study aims to investigate the mechanism of action of the cinnamon bark essential oil (CB), when used singly and also in combination with piperacillin, for its antimicrobial and synergistic activity against beta-lactamase TEM-1 plasmid-conferred Escherichia coli J53 R1. Viable count of bacteria for this combination of essential oil and antibiotic showed a complete killing profile at 20 h and further confirmed its synergistic effect by reducing the bacteria cell numbers. Analysis on the stability of treated cultures for cell membrane permeability by CB when tested against sodium dodecyl sulfate revealed that the bacterial cell membrane was disrupted by the essential oil. Scanning electron microscopy observation and bacterial surface charge measurement also revealed that CB causes irreversible membrane damage and reduces the bacterial surface charge. In addition, bioluminescence expression of Escherichia coli [pSB1075] and E. coli [pSB401] by CB showed reduction, indicating the possibility of the presence of quorum sensing (QS) inhibitors. Gas-chromatography and mass spectrometry of the essential oil of Cinnamomum verum showed that trans-cinnamaldehyde (72.81%), benzyl alcohol (12.5%), and eugenol (6.57%) were the major components in the essential oil. From this study, CB has the potential to reverse E. coli J53 R1 resistance to piperacillin through two pathways; modification in the permeability of the outer membrane or bacterial QS inhibition.