Contribution of Chloride Channel Conductance to the Regulation of Resting Membrane Potential in Chondrocytes
Contribution of Chloride Channel Conductance to the Regulation of Resting Membrane Potential in Chondrocytes
复制标题
DOI：
10.1254/jphs.10026sc
复制
发表时间：
2010-05-01
影响因子：
3.5
通讯作者：
Imaizumi, Yuji
中科院分区：
文献类型：
--
作者：
Funabashi, Kenji;Fujii, Masato;Imaizumi, Yuji
使用人类软骨细胞模型细胞系 OUMS-27 细胞检查了 Cl- 电导相对于 K+ 电导在膜电位调节中的贡献。 100 μM 尼氟酸 (NFA) 和其他阴离子通道阻滞剂的应用诱导了显着的膜超极化。电压钳下的NFA敏感膜电流主要是Cl-电流。 NFA的应用引起细胞内Ca2+浓度([Ca2+]i)小幅但显着的增加，并且显着增强了1μM组胺诱导的[Ca2+]i上升的晚期成分。总之，Cl-电导对 OUMS-27 细胞中静息膜电位和 [Ca2+]i 的调节有很大贡献。
The contribution of Cl- conductance relative to that of K+ in the regulation of membrane potential was examined using OUMS-27 cells, a model cell-line of human chondrocytes. Application of 100 mu M niflumic acid (NFA) and other anion-channel blockers induced significant membrane hyperpolarization. The NFA-sensitive membrane current under voltage-clamp was predominantly Cl- current. Application of NFA induced small but significant increase in intracellular Ca2+ concentration ([Ca2+]i) and markedly enhanced the late component of [Ca2+]i rise induced by 1 mu M histamine. In conclusion, Cl- conductance substantially contributes to the regulation of resting membrane potential and [Ca2+]i in OUMS-27 cells.