INVOLVEMENT OF GABA-A RECEPTORS IN THE OUTGROWTH OF CULTURED HIPPOCAMPAL-NEURONS
INVOLVEMENT OF GABA-A RECEPTORS IN THE OUTGROWTH OF CULTURED HIPPOCAMPAL-NEURONS
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DOI：
10.1016/0304-3940(93)90505-f
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发表时间：
1993-04-02
影响因子：
2.5
通讯作者：
BENARI, Y
中科院分区：
文献类型：
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作者：
BARBIN, G;POLLARD, H;BENARI, Y
虽然GABA是成年中枢神经系统中的主要抑制性神经递质，但我们实验室最近进行的实验表明，海马中GABA（A）受体的激活导致出生后早期的兴奋效应。GABA的去极化效应的可能后果进行了评估，胚胎海马神经元的神经炎生长在文化。当海马神经元在蝇蕈醇（GABA（A）受体激动剂）存在下培养三天时，未观察到形态学改变。与此相反，培养的海马神经元的神经炎生长的影响深远的存在荷包牡丹碱在培养基中。在这种GABA（A）受体拮抗剂的存在下，神经元显示出初级神经突和分支点数量的减少，导致总神经突长度的同时减少。因此，本研究表明，GABA作用于GABA（A）受体亚型，能够影响海马的发育。
Whereas GABA is a major inhibitory neurotransmitter in the adult central nervous system, recent experiments performed in our laboratory have shown that the activation of GABA(A) receptors in the hippocampus leads to excitatory effects during the early post-natal period. The possible consequence of a depolarizing effect of GABA was assessed on the neuritic outgrowth of embryonic hippocampal neurons in culture. No morphological alterations were observed when hippocampal neurons were cultured for three days in the presence of muscimol, a GABA(A) receptor agonist. In contrast, the neuritic outgrowth of cultured hippocampal neurons was profoundly affected by the presence of bicuculline in the culture medium. In the presence of this GABA(A) receptor antagonist neurons displayed a reduction in the number of primary neurites and branching points, resulting in a concomitant decrease of the total neuritic length. Thus, this study suggests that GABA acting on GABA(A) subtype of receptors, is able to affect the development of the hippocampus.