BLyS* and BLyS receptor expression in non-Hodgkin's lymphoma
BLyS* and BLyS receptor expression in non-Hodgkin's lymphoma
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DOI：
10.1016/s0301-472x(01)00774-3
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发表时间：
2002-02-01
影响因子：
2.6
通讯作者：
Levy, R
中科院分区：
文献类型：
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作者：
Briones, J;Timmerman, JM;Levy, R
目标。B淋巴细胞刺激因子(BLyS)蛋白及其受体是肿瘤坏死因子家族的新成员，对B细胞具有特异性作用。我们研究了不同类型非霍奇金淋巴瘤(NHL)患者肿瘤细胞BLyS受体(BLyS-R)的表达及血清BLyS蛋白水平。用流式细胞仪检测43例NHL患者和10例正常人B细胞BLyS-R的表达。用ELISA法检测血清BLyS蛋白水平。所有B细胞、肿瘤和正常组织均表达BLyS-R。BlyS-R在正常B细胞上的平均荧光强度(MFI+/-SD)为25.2+/-2.3个任意单位，而滤泡性非霍奇金淋巴瘤和慢性淋巴细胞白血病的BLyS-R表达水平显著低于正常B细胞(分别为17.7+/-3.1；15.5+/-3.9，P均为0.0001)，其他淋巴瘤亚型的表达水平与正常B细胞相当(弥漫性大细胞，24.8+/-4.3；套细胞，20+/-4.7；边缘带，20.7+/-3.7)。对15例正常人和17例滤泡性非霍奇金淋巴瘤患者血清BLyS蛋白水平进行了分析。滤泡性淋巴瘤患者的BlyS蛋白水平平均为正常人的3倍(平均+/-SD：13.4+/-5.6 ng/mLvs4.6+/-0.7 ng/mLp<；0.0001)。单独使用BLyS蛋白不能刺激滤泡性淋巴瘤B细胞和正常B细胞的增殖。B细胞淋巴瘤表达BLyS-R的特异性为靶向这种配体-受体对的治疗开辟了新的机会。(C)2002年国际实验血液学学会。爱思唯尔科学公司出版。
Objective. B Lymphocyte Stimulator (BLyS) protein and its receptor are new members of the tumor necrosis factor family, with specific effects exclusively on B cells. We have studied the tumor cell expression of the BLyS-Receptor (BLyS-R) and the serum BLyS protein levels in patients with different types of non-Hodgkin's lymphomas (NHL).Methods. BLyS-R expression was assessed by flow cytometry on B cells from 43 NHL patients and 10 normal donors. BLyS protein serum levels were analyzed by ELISA.Results. All B cells, tumor and normal, expressed BLyS-R. The mean fluorescence intensity (MFI +/- SD) of BLyS-R on normal B cells was 25.2 +/- 2.3 arbitrary units, while follicular NHL and chronic lymphocytic leukemia (CLL) exhibited significantly lower expression of the BLyS-R (17.7 +/- 3.1; 15.5 +/- 3.9, respectively, p < 0.0001 for both); other lymphoma subtypes expressed levels comparable to normal B cells (diffuse large cell, 24.8 +/- 4.3; mantle cell, 20 +/- 4.7; marginal zone, 20.7 +/- 3.7). BLyS protein serum levels were analyzed in 15 normal donors and 17 patients with follicular NHL. Levels of BLyS protein were, on average, threefold higher in patients with follicular lymphoma compared to normal donors (mean +/- SD; 13.4 +/- 5.6 ng/mL vs 4.6 +/- 0.7 ng/mL; p < 0.0001). BLyS protein alone was unable to stimulate proliferation in cultures of follicular lymphoma B cells or normal B cells.Conclusion. The specificity of the expression of BLyS-R by B-cell lymphomas opens new opportunities for the treatment of these cancers by targeting this ligand-receptor pair. (C) 2002 International Society for Experimental Hematology. Published by Elsevier Science Inc.