Micro RNA-373 is Down-regulated in Pancreatic Cancer and Inhibits Cancer Cell Invasion
Micro RNA-373 is Down-regulated in Pancreatic Cancer and Inhibits Cancer Cell Invasion
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DOI：
10.1245/s10434-014-3676-8
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发表时间：
2014-12-01
影响因子：
3.7
通讯作者：
Tanaka, Masao
中科院分区：
文献类型：
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作者：
Nakata, Kohei;Ohuchida, Kenoki;Tanaka, Masao
背景微小RNA（microRNAs，miRNAs）是一类非编码的小分子RNA，作为肿瘤恶性特征的关键分子而受到关注，最近的一些研究也发现了一些miRNAs作为抑制肿瘤恶性特征的潜在关键调控因子。最近有报道称，MiRNA-373（miR-373）可以诱导E-钙粘蛋白，而E-钙粘蛋白是上皮-间充质转化（EMT）的关键调节因子。然而，miR-373在癌细胞特性中的作用尚不清楚。我们通过体外和体内模型研究了miR-373在胰腺癌细胞系中的表达水平及其对胰腺癌侵袭力的影响。我们还使用福尔马林固定石蜡包埋（n = 152）和显微切割冷冻（n = 57）的胰腺组织样本分析了miR-373的表达。胰腺癌细胞系中miR-373的表达水平较低。在福尔马林固定石蜡包埋和显微切割的冷冻标本中，与健康胰腺相比，胰腺癌中miR-373的表达显著下调（P
Background. Micro RNAs (miRNAs) are small noncoding RNAs that have gained attention as key molecules in the malignant characteristics of cancers, and several recent investigations also have identified some miRNAs as potential key regulators to inhibit the malignant characteristics of tumors. MiRNA-373 (miR-373) has recently been reported to induce E-cadherin, which is a key regulator of epithelial-mesenchymal transition (EMT). However, the role of miR-373 in the characteristics of cancer cells is not still well known.Methods. We investigated the expression levels of miR-373 in pancreatic cancer cell lines and its effect on the invasiveness of pancreatic cancer by using in vitro and in vivo models. We also analyzed the expression of miR-373 using formalin-fixed paraffin-embedded (n = 152) and microdissected frozen (n = 57) samples from pancreatic tissues.Results. The levels of miR-373 expression were low in pancreatic cancer cell lines. In formalin-fixed paraffin-embedded and microdissected frozen samples, miR-373 expression was significantly down-regulated in pancreatic cancer compared with that in healthy pancreas (P