DNA microarrays: A novel approach to investigate genomics in trophoblast invasion - A review
DNA microarrays: A novel approach to investigate genomics in trophoblast invasion - A review
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DOI：
10.1053/plac.1999.0517
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发表时间：
2000-03-01
期刊：
影响因子：
3.8
通讯作者：
Quaranta, V
中科院分区：
文献类型：
--
作者：
Bilban, M;Head, S;Quaranta, V
调节滋养层侵袭的事件需要在转录水平上表征。几种类型的基因产物可能参与滋养层浸润的各个阶段，包括整合素、基质金属蛋白酶（MMP）和细胞外基质（ECM）蛋白。细胞滋养层增殖或分化的自分泌或旁分泌调节因子（如生长因子和细胞因子，以及氧张力）可以在转录水平上进行表征。大规模的基因表达谱的滋养层细胞的不同侵袭阶段，可以进行固定的组织通过激光定向显微切割。这一信息可能揭示了生理植入和胎盘，以及对病理过程，如先兆子痫的解释。DNA微阵列的应用是研究基因组结构（例如突变和多态性分析）和监测基因表达的理想选择。最终目标是在基因组水平上了解生长、发育、稳态、行为和疾病发生的关键事件。微阵列通过测量标记的单链DNA与固定在玻璃表面（"芯片"）上的数千个部分或全部基因序列的杂交来平行检测基因表达水平。微阵列既可商购获得，也可在内部制造。(C)2000年IFPA和Harcourt出版社
The events that regulate trophoblast invasion need to be characterized at the transcriptional level. Several types of gene products may be involved in various stages of trophoblast infiltration, including integrins, matrix metalloproteases (MMPs) and extracellular matrix (ECM) proteins. Autocrine or paracrine regulators of cytotrophoblast proliferation or differentiation in vitro (e.g. growth factors and cytokines, as well as oxygen tension) could be characterized mechanistically at the transcriptional level. Large-scale gene expression profiling of trophoblasts of distinct invasive stages could be carried out on fixed tissue obtained by laser-directed microdissection. This information may shed light on physiological implantation and placentation, as well as on the interpretation of pathological processes such as pre-eclampsia. The applications of DNA microarrays are ideal for studies of genomic structure (e.g. mutation and polymorphism analyses) and monitoring of gene expression. The ultimate goal is to understand the critical events underlying growth, development, homeostasis, behaviour and the onset of disease at a genomic level. Microarrays detect gene expression levels in parallel by measuring the hybridization of labelled, single-stranded DNA to many thousands of partial or whole gene sequences immobilized on a glass surface (the 'chip'). Microarrays are available both commercially and can be manufactured in house. (C) 2000 IFPA and Harcourt Publishers Ltd.