Analysis for selenium speciation in selenized yeast extracts by two-dimensional liquid chromatography with ICP-MS and electrospray MS-MS detection
Analysis for selenium speciation in selenized yeast extracts by two-dimensional liquid chromatography with ICP-MS and electrospray MS-MS detection
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采用 ICP-MS 和电喷雾 MS-MS 检测的二维液相色谱分析硒化酵母提取物中的硒形态
DOI：
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
R. Łobiński
中科院分区：
文献类型：
--
作者：
S. McSheehy;P. Pohl;J. Szpunar;M. Potin;R. Łobiński
开发了一种分析方法，可以检测以前未报道的谷胱甘肽S-共轭物与硒化合物的营养酵母补充剂的水提取物。该程序是基于使用二维尺寸排阻和反相HPLC分离的硒物种，ICP-MS的监测洗脱的硒，和电喷雾MS-MS的鉴定洗脱的物种。硒同位素模式的基础上确定的分子量为197，603，562，584，372和432（从反相HPLC洗脱顺序）的六种化合物的存在。通过碰撞诱导解离MS确认了Mr 197和432物种的身份，分别为硒代蛋氨酸和硒腺苷高半胱氨酸。通过与二硫苏糖醇反应，随后进行HPLC-ICP-MS和ES-MS-MS，证明具有较大Mr（562，584，604）的化合物含有谷胱甘肽（γGlu-Cys-Gly）和硒化合物之间的Se-S桥，该硒化合物在正ES-MS模式下没有响应。原始化合物的MS-MS分析证实了含硒片段的存在，该片段只能在高断裂能量下断裂。MS-MS证实，在372化合物中发现了一个类似的含硒部分（m/z 227），其抗碰撞诱导解离，与谷氨酰胺（Gln）残基连接。
An analytical approach allowing the detection of previously unreported glutathione S-conjugates with selenocompounds in water extracts of nutritional yeast supplements was developed. The procedure was based on the use of two-dimensional size-exclusion and reversed-phase HPLC for the separation of selenospecies, ICP-MS for the monitoring of the eluting selenium, and electrospray MS-MS for the identification of the eluted species. The presence of six compounds with molecular masses of 197, 603, 562, 584, 372 and 432 (in the elution order from reversed-phase HPLC) was identified on the basis of the selenium isotopic pattern. The identity of the Mr 197 and 432 species was confirmed, by collision induced dissociation MS, to be selenomethionine and Se-adenosylhomocysteine, respectively. The compounds with larger Mr (562, 584, 604) were demonstrated, by reaction with dithiothreitol followed by HPLC-ICP-MS and ES-MS-MS, to contain a Se–S bridge between glutathione (γGlu–Cys–Gly) and a selenocompound that did not respond in the positive ES-MS mode. The MS-MS analysis of the original compound confirmed the presence of a selenium-containing fragment that could be fragmented only at high fragmentation energies. A similar Se-containing moiety (m/z 227), resistant to collision induced dissociation, was found in the 372 compound attached to a glutamine (Gln) residue as demonstrated by MS-MS.
影响因子：
4.2
作者：
Kotrebai, M;Birringer, M;Uden, PC
通讯作者：
Uden, PC