Effects on protein synthesis produced by pairing depolarization with serotonin, an analogue of associative learning in Aplysia.
Effects on protein synthesis produced by pairing depolarization with serotonin, an analogue of associative learning in Aplysia.
复制标题
去极化与血清素配对对蛋白质合成的影响，血清素是海兔联想学习的类似物。
DOI：
10.1073/pnas.91.10.4150
复制
发表时间：
1994
影响因子：
11.1
通讯作者：
Eskin,A
中科院分区：
文献类型：
--
作者：
Noel,F;Koumenis,C;Nunez-Regueiro,M;Raju,U;Byrne,JH;Eskin,A
联想可塑性的一种形式，活动依赖性神经调节，涉及神经元活动的收敛和调节递质的作用。为了研究蛋白质合成在联想可塑性中的作用，我们研究了活性依赖性神经调节的生化类似物对标记氨基酸融入蛋白质水平的影响。为了模拟联想训练，腹部神经节暴露于去极化剂、高钾和调节递质血清素的配对治疗中。升高钾和血清素单独应用的影响也进行了检查。配对过程至少有两个蛋白（9号和17号）以非加性方式受到影响。结合到蛋白9的标签通过配对程序增加，但不受钾或血清素升高的影响。升高的钾或血清素对蛋白17中标签的掺入有显著影响，但联合使用的效果明显小于单独使用升高的钾和血清素的综合效果。这些结果表明，蛋白质合成的变化可能在联想可塑性的诱导中起重要作用。获得了蛋白9衍生的两个多肽的氨基酸序列。然后，用与这两个多肽部分序列对应的简并引物进行PCR，获得蛋白9的部分cDNA克隆。蛋白质9的序列与先前报道的一个基因家族的序列有关，该基因家族包括大肠杆菌的严格饥饿蛋白、植物的生长素诱导蛋白和谷胱甘肽s -转移酶。
A form of associative plasticity in Aplysia, activity-dependent neuromodulation, involves the convergence of neuronal activity and the effects of a modulatory transmitter. To investigate the role of protein synthesis in associative plasticity, we examined the effects of a biochemical analogue of activity-dependent neuromodulation on the level of incorporation of labeled amino acid into proteins. To mimic associative training, abdominal ganglia were exposed to paired treatments of a depolarizing agent, elevated potassium, and a modulatory transmitter, serotonin. The effects of elevated potassium and serotonin applied alone were also examined. At least two proteins (nos. 9 and 17) were affected in a nonadditive way by the paired procedure. Incorporation of label into protein 9 was increased by the paired procedure but was not affected by either elevated potassium or serotonin. Incorporation of label into protein 17 was significantly affected by elevated potassium or serotonin, but the effect of the paired procedure was significantly less than the summed effects of elevated potassium and serotonin applied alone. These results indicate that changes in protein synthesis may be important in the induction of associative plasticities. Amino acid sequences of two peptides derived from protein 9 were obtained. Then, a partial cDNA clone for protein 9 was obtained by performing PCR with degenerate primers corresponding to portions of the sequences of the two peptides. The sequence of protein 9 is related to sequences previously reported for a family of genes comprising the stringent starvation protein of Escherichia coli, auxin-induced proteins of plants, and glutathione S-transferases of a number of organisms.