An ABCC-type transporter endowing glyphosate resistance in plants
An ABCC-type transporter endowing glyphosate resistance in plants
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赋予植物草甘膦抗性的 ABCC 型转运蛋白
DOI：
10.1073/pnas.2100136118
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发表时间：
2021-04-20
影响因子：
11.1
通讯作者：
Powles, Stephen
中科院分区：
文献类型：
--
作者：
Pan, Lang;Yu, Qin;Powles, Stephen
草甘膦是世界农业中使用最广泛的除草剂，在各种情况下用于一般植被控制。在全球范围内，对大型杂草植物群体进行密集的草甘膦选择，导致了许多杂草物种群体中广泛的草甘膦抗性进化。在这里，我们研究了在西澳大利亚农业领域进化的抗草甘膦（GR）棘皮藻种群，发现了一种atp结合盒（ABC）转运体（EcABCC8），该转运体在GR植物中持续上调。当在转基因水稻中表达时，该EcABCC8转运体赋予了草甘膦抗性。同样，过量表达EcABCC8同源基因的水稻、玉米和大豆也对草甘膦产生抗性。相反，CRISPR/ cas9介导的敲除EcABCC8同源基因OsABCC8增加了水稻对草甘膦的敏感性。在ABCC8转基因水稻植株和分离的叶片原生质体中，亚细胞定位分析和草甘膦细胞水平定量以及结构建模支持了EcABCC8可能是一种质膜定位转运体，将细胞质草甘膦挤压到外质体，降低细胞草甘膦水平。这是一篇在GR植物中外排草甘膦的膜转运蛋白的报道，其功能可能在作物植物中是保守的。
Glyphosate is the most widely used herbicide in world agriculture and for general vegetation control in a wide range of situations. Global and often intensive glyphosate selection of very large weedy plant populations has resulted in widespread glyphosate resistance evolution in populations of many weed species. Here, working with a glyphosate-resistant (GR) Echinochloa colona population that evolved in a Western Australia agricultural field, we identified an ATP-binding cassette (ABC) transporter (EcABCC8) that is consistently up-regulated in GR plants. When expressed in transgenic rice, this EcABCC8 transporter endowed glyphosate resistance. Equally, rice, maize, and soybean overexpressing the EcABCC8 ortholog genes were made resistant to glyphosate. Conversely, CRISPR/Cas9-mediated knockout of the EcABCC8 ortholog gene OsABCC8 increased rice susceptibility to glyphosate. Subcellular localization analysis and quantification of glyphosate cellular levels in treated ABCC8 transgenic rice plants and isolated leaf protoplasts as well as structural modeling support that EcABCC8 is likely a plasma membrane-localized transporter extruding cytoplasmic glyphosate to the apoplast, lowering the cellular glyphosate level. This is a report of a membrane transporter effluxing glyphosate in a GR plant species, and its function is likely conserved in crop plant species.